GAPDHS antibody (FITC)

Catalog No. ABIN1302845

The Mouse Monoclonal anti-GAPDHS antibody has been validated for FACS. It is suitable to detect GAPDHS in samples from Human and Pig.

Quick Overview for GAPDHS antibody (FITC) (ABIN1302845)

Target: GAPDHS (Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS))

Reactivity: Human, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This GAPDHS antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: Hs-8

Product Details anti-GAPDHS Antibody (FITC)

Purpose: Anti-GAPDHS FITC

Specificity: The antibody Hs-8 reacts with GAPDHS, the sperm-specific glyceraldehyde phosphate dehydrogenase, which is an intra-acrosomal protein.

Cross-Reactivity (Details): Human, Porcine

Purification: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.

Isotype: IgM

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 50 tests. Intraacrosomal staining.

Restrictions: For Research Use only

Handling

Buffer: Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid prolonged exposure to light.
Do not use after expiration date stamped on vial label.
Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Target Details for GAPDHS

Target: GAPDHS (Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS))

Alternative Name: GAPDHS

Background: Glyceraldehyde-3-phosphate dehydrogenase, spermato,GAPDHS (the sperm-specific glyceraldehyde phosphate dehydrogenase, also known as GAPD2, GAPDS, HSD-35, or GAPDH-2, is a glycolytic enzyme that plays an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. It can be used as an intra-acrosomal marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.,SGAPDH, HSD-35

Gene ID: 26330

UniProt: O14556

Pathways: Regulation of Carbohydrate Metabolic Process