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GAPDHS antibody

GAPDHS Reactivity: Human, Pig WB, FACS, ICC Host: Mouse Monoclonal Hs-8 unconjugated
Catalog No. ABIN94395
  • Target See all GAPDHS Antibodies
    GAPDHS (Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS))
    Reactivity
    • 23
    • 18
    • 17
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    Human, Pig
    Host
    • 31
    • 7
    • 1
    Mouse
    Clonality
    • 33
    • 6
    Monoclonal
    Conjugate
    • 17
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This GAPDHS antibody is un-conjugated
    Application
    • 31
    • 13
    • 13
    • 7
    • 6
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)
    Specificity
    The antibody Hs-8 reacts with GAPDHS, the sperm-specific glyceraldehyde phosphate dehydrogenase, which is an intra-acrosomal protein.
    Cross-Reactivity (Details)
    Human, Porcine
    Purification
    Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
    Purity
    > 95 % (by SDS-PAGE)
    Immunogen
    Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
    Clone
    Hs-8
    Isotype
    IgM
    Top Product
    Discover our top product GAPDHS Primary Antibody
  • Application Notes
    Immunocytochemistry: Recommended dilution: 10 μg/mL, membrane permeabilization (acetone) is essential.
    Flow cytometry: Recommended dilution: 3-12 μg/mL. Intraacrosomal staining.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Do not freeze.
  • Target
    GAPDHS (Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS))
    Alternative Name
    GAPDHS (GAPDHS Products)
    Synonyms
    GAPDS antibody, GAPDHS antibody, GAPD2 antibody, GAPDH-2 antibody, HSD-35 antibody, Gapd-s antibody, Gapds antibody, gapdh-2 antibody, cb350 antibody, fb71f08 antibody, fk58c09 antibody, g3pdh antibody, gapdh antibody, gapds antibody, wu:fb71f08 antibody, wu:fk58c09 antibody, zgc:76908 antibody, glyceraldehyde-3-phosphate dehydrogenase, spermatogenic antibody, GAPDHS antibody, Gapdhs antibody, gapdhs antibody
    Background
    Glyceraldehyde-3-phosphate dehydrogenase, spermato,GAPDHS (the sperm-specific glyceraldehyde phosphate dehydrogenase, also known as GAPD2, GAPDS, HSD-35, or GAPDH-2, is a glycolytic enzyme that plays an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. It can be used as an intra-acrosomal marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.,SGAPDH, HSD-35
    Gene ID
    26330
    UniProt
    O14556
    Pathways
    Regulation of Carbohydrate Metabolic Process
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