KCNA1 antibody (Extracellular)

Catalog No. ABIN1304744

There are 28+ publications for this product available. The Mouse Monoclonal anti-KCNA1 antibody is suitable to detect KCNA1 in samples from Rat. It has been validated for WB, ELISA, IHC and ICC.

Quick Overview for KCNA1 antibody (Extracellular) (ABIN1304744)

Target: KCNA1 (Potassium Voltage-Gated Channel, Shaker-Related Subfamily, Member 1 (KCNA1))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This KCNA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Grade: KO Validated

Clone: K36-15

Product Details anti-KCNA1 Antibody

Binding Specificity: AA 191-208, Extracellular

Purpose: Anti-Kv1.1 K+ Channel (External) Antibody

Sequence: ELKDDKDFTG TIHRIDNTC

Specificity: No off-targets reported

Cross-Reactivity: Mouse

Characteristics: Our Anti-Kv1.1 K+ channel (external) mouse monoclonal primary antibody is produced in-house from hybridoma clone K36/15. It is KO validated, detects mouse and rat Kv1.1 K+ channel (external), and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, ELISA.

Purification: Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Purity: Purified mAbs are >90 % specific antibody.

Immunogen: Synthetic peptide amino acids 191-208 (ELKDDKDFTGTIHRIDNTC, extracellular domain) of rat Kv1.1 (accession number P10499)

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 10 mM Tris, 50 mM Sodium Chloride, 0.065 % Sodium Azide 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Expiry Date: 24 months

References for anti-KCNA1 antibody (ABIN1304744)

Chen, Koopmans, Gordon, Paliukhovich, Klaassen, van der Schors, Peles, Verhage, Smit, Li: "Interaction proteomics of canonical Caspr2 (CNTNAP2) reveals the presence of two Caspr2 isoforms with overlapping interactomes." in: Biochimica et biophysica acta, Vol. 1854, Issue 7, pp. 827-33, (2015) (PubMed).

Zollinger, Chang, Baalman, Kim, Rasband: "The Polarity Protein Pals1 Regulates Radial Sorting of Axons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 29, pp. 10474-84, (2015) (PubMed).

Sosanya, Brager, Wolfe, Niere, Raab-Graham: "Rapamycin reveals an mTOR-independent repression of Kv1.1 expression during epileptogenesis." in: Neurobiology of disease, Vol. 73, pp. 96-105, (2014) (PubMed).

Jones, Corbin, Huntsman: "Neonatal NMDA receptor blockade disrupts spike timing and glutamatergic synapses in fast spiking interneurons in a NMDA receptor hypofunction model of schizophrenia." in: PLoS ONE, Vol. 9, Issue 10, pp. e109303, (2014) (PubMed).

Hoppa, Gouzer, Armbruster, Ryan: "Control and plasticity of the presynaptic action potential waveform at small CNS nerve terminals." in: Neuron, Vol. 84, Issue 4, pp. 778-89, (2014) (PubMed).

Kirizs, Kerti-Szigeti, Lorincz, Nusser: "Distinct axo-somato-dendritic distributions of three potassium channels in CA1 hippocampal pyramidal cells." in: The European journal of neuroscience, Vol. 39, Issue 11, pp. 1771-83, (2014) (PubMed).

Desmazieres, Zonta, Zhang, Wu, Sherman, Brophy: "Differential stability of PNS and CNS nodal complexes when neuronal neurofascin is lost." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 34, Issue 15, pp. 5083-8, (2014) (PubMed).

Dufour, Woodhouse, Goaillard: "Somatodendritic ion channel expression in substantia nigra pars compacta dopaminergic neurons across postnatal development." in: Journal of neuroscience research, Vol. 92, Issue 8, pp. 981-99, (2014) (PubMed).

Campanac, Gasselin, Baude, Rama, Ankri, Debanne: "Enhanced intrinsic excitability in basket cells maintains excitatory-inhibitory balance in hippocampal circuits." in: Neuron, Vol. 77, Issue 4, pp. 712-22, (2013) (PubMed).

Lallet-Daher, Wiel, Gitenay, Navaratnam, Augert, Le Calvé, Verbeke, Carling, Aubert, Vindrieux, Bernard: "Potassium channel KCNA1 modulates oncogene-induced senescence and transformation." in: Cancer research, Vol. 73, Issue 16, pp. 5253-65, (2013) (PubMed).

Sosanya, Huang, Cacheaux, Chen, Nguyen, Perrone-Bizzozero, Raab-Graham: "Degradation of high affinity HuD targets releases Kv1.1 mRNA from miR-129 repression by mTORC1." in: The Journal of cell biology, Vol. 202, Issue 1, pp. 53-69, (2013) (PubMed).

Kyle, Ahrendt, Braun, Braun: "The large conductance, calcium-activated K+ (BK) channel is regulated by cysteine string protein." in: Scientific reports, Vol. 3, pp. 2447, (2013) (PubMed).

Liu, Lee, Davis: "Heterogeneous intrinsic excitability of murine spiral ganglion neurons is determined by Kv1 and HCN channels." in: Neuroscience, Vol. 257, pp. 96-110, (2013) (PubMed).

Bildl, Haupt, Müller, Biniossek, Thumfart, Hüber, Fakler, Schulte: "Extending the dynamic range of label-free mass spectrometric quantification of affinity purifications." in: Molecular & cellular proteomics : MCP, Vol. 11, Issue 2, pp. M111.007955, (2012) (PubMed).

Li, Lu, Xu, Zhang, Zhu, Zhang, Cao, Chen, Chen, Luo, Duan, Li: "Neuregulin 1 regulates excitability of fast-spiking neurons through Kv1.1 and acts in epilepsy." in: Nature neuroscience, Vol. 15, Issue 2, pp. 267-73, (2012) (PubMed).

Bangratz, Sarrazin, Devaux, Zambroni, Echaniz-Laguna, René, Boërio, Davoine, Fontaine, Feltri, Benoit, Nicole et al.: "A mouse model of Schwartz-Jampel syndrome reveals myelinating Schwann cell dysfunction with persistent axonal depolarization in vitro and distal peripheral nerve hyperexcitability when perlecan is ..." in: The American journal of pathology, Vol. 180, Issue 5, pp. 2040-55, (2012) (PubMed).

Irani, Pettingill, Kleopa, Schiza, Waters, Mazia, Zuliani, Watanabe, Lang, Buckley, Vincent: "Morvan syndrome: clinical and serological observations in 29 cases." in: Annals of neurology, Vol. 72, Issue 2, pp. 241-55, (2012) (PubMed).

Zhu, Alsaber, Zhao, Ribeiro-Hurley, Thornhill: "Characterization of the Kv1.1 I262T and S342I mutations associated with episodic ataxia 1 with distinct phenotypes." in: Archives of biochemistry and biophysics, Vol. 524, Issue 2, pp. 99-105, (2012) (PubMed).

Menegola, Clark, Trimmer: "The importance of immunohistochemical analyses in evaluating the phenotype of Kv channel knockout mice." in: Epilepsia, Vol. 53 Suppl 1, pp. 142-9, (2012) (PubMed).

Giacomini, La Padula, Schenone, Leandri, Contestabile, Moruzzo, Goutebroze, Consalez, Benfenati, Corradi: "Both Schwann cell and axonal defects cause motor peripheral neuropathy in Ebf2-/- mice." in: Neurobiology of disease, Vol. 42, Issue 1, pp. 73-84, (2011) (PubMed).

Target Details for KCNA1

Target: KCNA1 (Potassium Voltage-Gated Channel, Shaker-Related Subfamily, Member 1 (KCNA1))

Alternative Name: Kv1.1 K+ channel

Background:

Synonyms: Potassium voltage-gated channel subfamily A member 1 (RBKI) (RCK1) (Voltage-gated potassium channel subunit Kv1.1)

Description Logic: Our Anti-Kv1.1 K+ channel (external) mouse monoclonal primary antibody is produced from hybridoma clone K36/15. It is KO validated, detects mouse and rat Kv1.1 K+ channel (external), and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, ELISA.

Gene Name Alternatives: Kcna1

Molecular Weight: 56 kDa (could be 65-85 kDa depending on glycosylation)

UniProt: P10499