HSPA9 antibody (AA 551-766)

Catalog No. ABIN1304832

This anti-HSPA9 antibody is a Mouse Monoclonal antibody detecting HSPA9 in WB, IHC, ICC and IP. Suitable for Mouse.

Quick Overview for HSPA9 antibody (AA 551-766) (ABIN1304832)

Target: HSPA9 (Heat Shock 70kDa Protein 9 (Mortalin) (HSPA9))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This HSPA9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Clone: N52A-42

Product Details anti-HSPA9 Antibody

Binding Specificity: AA 551-766

Specificity: No off-targets reported

Cross-Reactivity: Human, Mouse, Non-Human Primate, Rat

Characteristics:

Description: Our Anti-Mortalin/GRP75 mouse monoclonal primary antibody is produced in-house from hybridoma clone N52A/42. It detects human, mouse, and rat Mortalin/GRP75, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Manufacturer Comment: We produce our Mortalin/GRP75 mouse monoclonal primary antibody from hybridoma clone N52A/42. It is great in IHC, ICC, IP, WB and is purified by Protein A chromatography.

Purification: Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography.

Purity: > 90% specific antibody

Immunogen: Identified as off-target mAb in screen for anti-Salm2 NeuroMabs (immunogen was fusion protein amino acids 551-766 of mouse SALM2). Target identified as Mortalin/Grp75 by LC-MS/MS analysis of protein immunopurified from rat brain using N52A/42.

Isotype: IgG1

Application Details

Application Notes:

Dilution Range: ICC: 1:200

Dilution Range: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 10 mM Tris, 50 mM Sodium Chloride, 0.065 % Sodium Azide pH 7.4

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Expiry Date: 24 months

Target Details for HSPA9

Target: HSPA9 (Heat Shock 70kDa Protein 9 (Mortalin) (HSPA9))

Alternative Name: GRP75

Background:

Synonyms: Stress-70 protein, mitochondrial (75 kDa glucose-regulated protein) (GRP-75) (Heat shock 70 kDa protein 9) (Mortalin) (Peptide-binding protein 74) (PBP74) (p66 MOT)

Target Description: Mitochondrial stress-70 protein or Mortalin/GRP75 is encoded by the gene HSPA9. Mortalin is a chaperone protein and plays a role in cell proliferation, stress response and mitochondria maintenance. It is widely expressed in tissues in mitochondria, endoplasmic reticulum, plasma membrane and cytoplasmic vesicles. Diseases associated with this gene include Even-Plus Syndrome and Sideroblastic Anemia 4.

Gene Name Alternatives: Hspa9 Grp75 Hsp74 Hspa9a

Molecular Weight: 75 kDa

UniProt: P38647