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COL2 antibody (Biotin)

This Goat Polyclonal antibody specifically detects COL2 in ELISA. It exhibits reactivity toward Human.
Catalog No. ABIN135049

Quick Overview for COL2 antibody (Biotin) (ABIN135049)

Target

See all COL2 Antibodies
COL2 (Collagen, Type II (COL2))

Reactivity

  • 48
  • 40
  • 37
  • 27
  • 19
  • 12
  • 9
  • 1
  • 1
  • 1
Human

Host

  • 49
  • 13
  • 9
  • 7
Goat

Clonality

  • 65
  • 13
Polyclonal

Conjugate

  • 38
  • 14
  • 4
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This COL2 antibody is conjugated to Biotin

Application

  • 58
  • 36
  • 25
  • 21
  • 16
  • 15
  • 15
  • 13
  • 13
  • 13
  • 7
  • 6
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
ELISA
  • Specificity

    Reacts with conformational determinants on type II collagen Referenced species reactivities include - Human 4,5,10,12,14,18-20,22,24,27,29,31,33,34 Chicken 16,26 Mouse 6,7,15 Porcine 21 Rat 13,17,25 Rabbit 2,9,11,30,32 Bovine 2,3,19,23 Sheep 8,28

    Cross-Reactivity (Details)

    Exhibits < 10 % cross reactivity with collagen type II, III, IV, V and VI. The antibody has not been tested for reactivity with other ECM proteins (e.g., laminin, fibronectin).

    Characteristics

    Goat Anti-Type II Collagen-BIOT

    Purification

    Purification Method: Affinity chromatography on type II collagen covalently linked to agarose.

    Isotype

    IgG
  • Application Notes

    • Applications: Quality tested applications include - ELISA , FLISA
    • Other referenced applications include - IHC-PS , IHC-FS , ICC , EM , WB , IP
    • Working Dilutions: ELISA BIOT conjugate 1:1,000 - 1:4,000 FLISA AF488 conjugate 1:200 - 1:400

    Sample Volume

    0,5 mL

    Restrictions

    For Research Use only
  • Concentration

    0.4 mg/mL

    Buffer

    0.2 mg in 0.5 mL of PBS/Sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Protect conjugated products from light.
    Each reagent is stable for the period shown on the bottle label if stored as directed.

    Storage

    4 °C

    Storage Comment

    Store at 2-8°C
  • Target

    COL2 (Collagen, Type II (COL2))

    Alternative Name

    Type II Collagen
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