COL3 antibody (Biotin)

Catalog No. ABIN135051

Cited in 2+ publications. The Biotin-conjugated Goat Polyclonal anti-COL3 antibody (ABIN135051) specifically detects COL3 in WB, ELISA, IHC (fro), ICC, IHC (p), FACS and IEM. The antibody is reactive with Human, Rat, Mouse, Dog, Rabbit, Chicken, Cow, Chinchilla chinchilla, Guinea Pig, Hamster, Horse, Pig, Rhesus Monkey and Tree shrew samples.

Quick Overview for COL3 antibody (Biotin) (ABIN135051)

Target: COL3 (Collagen, Type III (COL3))

Reactivity: Human, Rat, Mouse, Dog, Rabbit, Chicken, Cow, Chinchilla chinchilla, Guinea Pig, Hamster, Horse, Pig, Rhesus Monkey, Tree shrew

Host: Goat

Clonality: Polyclonal

Conjugate: This COL3 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunoelectron Microscopy (IEM)

Product Details anti-COL3 Antibody (Biotin)

Purpose: Goat Anti-Type III Collagen-BIOT

Specificity: Reacts with conformational determinants on type III collagen Referenced species reactivities include - Human 2,5,11,12,32,33,35-37 Chicken 22,23 Hamster 18,24 Mouse 4,6,13,15,34,39 Chinchilla 26 Porcine 1,5,17,29 Rat 5,10,14,20,31 Equine 25 Rabbit 9,16,19,21,38 Bovine 8,27 Feline 30 Rhesus 7 Canine 3,5 Guinea Pig 21 Tree Shrew 28

Cross-Reactivity (Details): Exhibits < 10 % cross reactivity with collagen type II, III, IV, V and VI. The antibody has not been tested for reactivity with other ECM proteins (e.g., laminin, fibronectin).

Characteristics: Biotin Anti-Type III Collagen antibody for use in ELISA, immunohistochemistry / immunocytochemistry, electron microscopy, flow cytometry, and western blotting assays.

Purification: Purified by affinity chromatography on type III collagen covalently linked to agarose, cross-adsorbed against Collagen types I, II, IV, V, and VI for minimal reactivity

Immunogen: Type III collagen

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Pooled antisera from goats hyperimmunized with type III collagen

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.4 mg/mL

Buffer: Phosphate buffered saline containing < 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8°C

References for anti-COL3 antibody (Biotin) (ABIN135051)

Smeland, Lu, Karlsen, Salvesen, Reed, Stuhr: "Stromal integrin α11-deficiency reduces interstitial fluid pressure and perturbs collagen structure in triple-negative breast xenograft tumors." in: BMC cancer, Vol. 19, Issue 1, pp. 234, (2019) (PubMed).

Buckley, Evans, Matuszewski, Chen, Satchel, Elliott, Soslowsky, Dodge: "Distributions of types I, II and III collagen by region in the human supraspinatus tendon." in: Connective tissue research, Vol. 54, Issue 6, pp. 374-9, (2014) (PubMed).

Target Details for COL3

Target: COL3 (Collagen, Type III (COL3))

Alternative Name: Type III Collagen

Background:

Description: Goat Anti-Type III Collagen-BIOT

NCBI_Official_Symbol: COL3A1

Synoynms: COL3

Gene ID: 1281

UniProt: P02461