Phosphotyrosine antibody

Catalog No. ABIN135058

This Mouse Monoclonal antibody specifically detects Phosphotyrosine in ELISA.

Quick Overview for Phosphotyrosine antibody (ABIN135058)

Target: Phosphotyrosine

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This Phosphotyrosine antibody is un-conjugated

Application: ELISA

Clone: PY20

Product Details anti-Phosphotyrosine Antibody

Specificity: Tyrosine-phosphorylated proteins

Characteristics: Mouse Anti-Phosphotyrosine-UNLB

Purification: Purified

Immunogen: Phosphotyrosine conjugated to carrier proteins

Isotype: IgG2b

Application Details

Application Notes:

• Applications: ELISA - Quality tested , FLISA - Quality tested WB - Reported in literature , IP - Reported in literature , IHC-PS - Reported in literature , IHC-WM - Reported in literature , ICC - Reported in literature , FC - Reported in literature , Microarray - Reported in literature , Purification - Reported in literature
• Working Dilutions: ELISA AP conjugate 1:1,000 - 1:2,000 HRP conjugate 1:1,000 - 1:4,000 BIOT conjugate 1:5,000 - 1:10,000 FLISA FITC conjugate 1:200 - 1:400 PE conjugate ≤ 1 g/mL

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Preservative: Without preservative

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for Phosphotyrosine

Target: Phosphotyrosine

Target Type: Amino Acid

Background: Protein tyrosine residues are phosphorylated as a result of intracellular protein kinase activation (e.g. via growth factors) during normal growth and development, and in oncogenesis. The most abundant population of target proteins for tyrosine phosphorylation are cell surface glycoproteins. Antibody to phosphotyrosine enables the detection, isolation and characterization of proteins containing phosphotyrosine. PY20 also prevents internalization of activated receptors, e.g. EGFr, when microinjected into cells. The affinity of PY20 for phosphotyrosine is approximately 10-6 to 10-7 M. PY20 binding to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate, but not by phosphoserine, phosphothreonine or free phosphate.