Phosphotyrosine antibody (HRP)

Catalog No. ABIN135060

The HRP-conjugated Mouse Monoclonal anti-Phosphotyrosine antibody (Clone PY20) (ABIN135060) specifically detects Phosphotyrosine in WB, IHC and ELISA.

Quick Overview for Phosphotyrosine antibody (HRP) (ABIN135060)

Target: Phosphotyrosine

Host: Mouse

Clonality: Monoclonal

Conjugate: This Phosphotyrosine antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: PY20

Product Details anti-Phosphotyrosine Antibody (HRP)

Characteristics: The antibody detects: Phosphorylated tyrosine and phenylphosphate

Purification: Purified

Isotype: IgG2b

Application Details

Application Notes: Working Dilution:
ELISA: 1:1,000-1:4,000
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Product is supplied as 1.0 mL of stock solution in 50 % glycerol / 50 % PBS, pH 7.4.

Handling Advice: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Target Details for Phosphotyrosine

Target: Phosphotyrosine

Target Type: Amino Acid

Background: Protein tyrosine residues are phosphorylated as a result of intracellular protein kinase activation (e.g. via growth factors) during normal growth and development, and in oncogenesis. The most abundant population of target proteins for tyrosine phosphorylation are cell surface glycoproteins. Antibody to phosphotyrosine enables the detection, isolation and characterization of proteins containing phosphotyrosine. PY20 also prevents internalization of activated receptors, e.g. EGFr, when microinjected into cells. The affinity of PY20 for phosphotyrosine is approximately 10-6 to 10-7 M. PY20 binding to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate, but not by phosphoserine, phosphothreonine or free phosphate.