CD44 antibody

Catalog No. ABIN135065

The Rat Monoclonal anti-CD44 antibody has been validated for FACS. It is suitable to detect CD44 in samples from Mouse. There are 3+ publications available.

Quick Overview for CD44 antibody (ABIN135065)

Target: CD44

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD44 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: KM201

Product Details anti-CD44 Antibody

Specificity: Mouse CD44 (all isoforms), Mr 80-95 kDa

Characteristics: Rat Anti-Mouse CD44-UNLB

Purification: Purified

Immunogen: (C57BL/6 x DBA/2)F1 mouse bone marrow-derived stromal clone BMS2

Isotype: IgG1

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , ICC - Reported in literature , IP - Reported in literature , WB - Reported in literature , Block - Reported in literature , Adhesion - Reported in literature
• Working Dilutions: Flow Cytometry FITC and BIOT conjugates 2 g/106 cells PE, APC, and SPRD conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

cell adhesion and homing

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Preservative: Without preservative

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References for anti-CD44 antibody (ABIN135065)

Rux, Song, Swinehart, Pineault, Schlientz, Trulik, Goldstein, Kozloff, Lucas, Wellik: "Regionally Restricted Hox Function in Adult Bone Marrow Multipotent Mesenchymal Stem/Stromal Cells." in: Developmental cell, Vol. 39, Issue 6, pp. 653-666, (2017) (PubMed).

Goldman, Majumder, Dhawan, Ravi, Goldman, Kohandel, Majumder, Sengupta: "Temporally sequenced anticancer drugs overcome adaptive resistance by targeting a vulnerable chemotherapy-induced phenotypic transition." in: Nature communications, Vol. 6, pp. 6139, (2016) (PubMed).

Sánchez, Hill, Love, Soslow, Craig, Austin, Brown, Czirok, Camenisch, Barnett: "The cytoplasmic domain of TGFβR3 through its interaction with the scaffolding protein, GIPC, directs epicardial cell behavior." in: Developmental biology, Vol. 358, Issue 2, pp. 331-43, (2011) (PubMed).

Target Details for CD44

Target: CD44

Alternative Name: CD44

Background: CD44 represents a family of type I transmembrane glycoproteins expressed on many cell types such as B cells, monocytes, macrophages, and variable subsets of thymocytes and peripheral T cells. CD44 primarily functions as an adhesion molecule. It has been implicated in various processes, such as hematopoiesis, lymphocyte homing, leukocyte activation, tumor metastasis and development. The KM201 monoclonal antibody reacts with an epitope very close to the hyaluronate binding domain on the CD44 molecule. KM201 can inhibit hyaluronate-dependent cell aggregation, prevent lympho-hemopoiesis in both Dexter and Whitlock-Witte Cultures, prevent the earliest intrathymic precursors from homing to the thymus, and costimulate the activation of freshly purified splenic CD4+ T cells.

Pathways: Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling