VCAM1 antibody

Catalog No. ABIN135070

This Rat Monoclonal antibody specifically detects VCAM1 in FACS. It exhibits reactivity toward Mouse and has been mentioned in 3+ publications.

Quick Overview for VCAM1 antibody (ABIN135070)

Target: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This VCAM1 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: M-K-2

Product Details anti-VCAM1 Antibody

Specificity: Mouse CD106, Mr 100-110 kDa

Characteristics: Rat Anti-Mouse CD106-UNLB

Purification: Purified

Immunogen: BALB/3T3 and +/+2.4 cells

Isotype: IgG1

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature , WB - Reported in literature , Adhesion - Reported in literature , Block - Reported in literature
• Working Dilutions: Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Preservative: Without preservative

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References for anti-VCAM1 antibody (ABIN135070)

Wang, Cao, Gorshkov, Zhou, Yang, Xu, Ma, Zhang, Wang, Mao, Zeng, Su, Verin, Hong, Liu, Huo: "Ablation of endothelial Pfkfb3 protects mice from acute lung injury in LPS-induced endotoxemia." in: Pharmacological research, Vol. 146, pp. 104292, (2020) (PubMed).

Xu, Wang, Yan, Yang, Zhou, Zeng, Liu, An, Toque, Dong, Jiang, Fulton, Weintraub, Li, Bagi, Hong, Boison, Wu, Huo: "Regulation of endothelial intracellular adenosine via adenosine kinase epigenetically modulates vascular inflammation." in: Nature communications, Vol. 8, Issue 1, pp. 943, (2018) (PubMed).

Siler, Berlow, Kukino, Davis, Nelson, Grafe, Ono, Cetas, Pike, Alkayed: "Soluble Epoxide Hydrolase in Hydrocephalus, Cerebral Edema, and Vascular Inflammation After Subarachnoid Hemorrhage." in: Stroke, Vol. 46, Issue 7, pp. 1916-22, (2015) (PubMed).

Target Details for VCAM1

Target: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Alternative Name: CD106

Background: VCAM-1 is an adhesion molecule and a major mediator of the inflammatory response. It is expressed on activated microvascular endothelial cells in response to signals arising from immune responses in infection, graft rejection, tumor recognition and killing. The complementary binding ligand for VCAM-1 is VLA-4/CD49d. In addition to VCAM-1, VLA-4 also recognizes the extracellular matrix molecule fibronectin. This pairing of VCAM-1 and VLA-4 is able to provide a second signal (i.e., non-antigen specific) for T cell stimulation, such as that seen in transplantation. The monoclonal antibody MK-2 has been used in transplant studies to suppress cardiac rejection and induce long-term cardiac graft survival. In addition to inflammatory responses, VCAM-1 has a significant role in hemopoiesis through its ability to retain lymphocyte and myeloid precursors on stromal cells in the marrow and lymphoid organs. CD106/VCAM-1 exists as an integral membrane protein.

Pathways: Carbohydrate Homeostasis