VCAM1 antibody

Catalog No. ABIN135074

There are 2+ publications for this product available. The Rat Monoclonal anti-VCAM1 antibody is suitable to detect VCAM1 in samples from Mouse. It has been validated for WB, FACS, IP, IHC (fro), BR and Adh Act.

Quick Overview for VCAM1 antibody (ABIN135074)

Target: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This VCAM1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Reagent (BR), Adhesion Activation (Adh Act)

Clone: M-K-2

Product Details anti-VCAM1 Antibody

Purpose: Rat Anti-Mouse CD106-LE/AF

Specificity: Mouse CD106, Mr 100-110 kDa

Characteristics: Low Endotoxin/Azide Free Anti-Mouse CD106 antibody for use in flow cytometry, immunohistochemistry, western blotting, immunoprecipitation, blocking, and adhesion assays.

Endotoxin Level: Low Endotoxin

Immunogen: BALB/3T3 and +/+2.4 cells

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Phosphate buffered saline, pH 7.4

Preservative: Azide free

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: 2-8°C

References for anti-VCAM1 antibody (ABIN135074)

Gilchrist, Kinchesh, Zarghami, Khrapitchev, Sibson, Kersemans, Smart: "Improved detection of molecularly targeted iron oxide particles in mouse brain using B0 field stabilised high resolution MRI." in: Magnetic resonance imaging, Vol. 67, pp. 101-108, (2020) (PubMed).

Zarghami, Khrapitchev, Perez-Balderas, Soto, Larkin, Bau, Sibson: "Optimization of molecularly targeted MRI in the brain: empirical comparison of sequences and particles." in: International journal of nanomedicine, Vol. 13, pp. 4345-4359, (2018) (PubMed).

Target Details for VCAM1

Target: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Alternative Name: CD106

Background: VCAM-1 is an adhesion molecule and a major mediator of the inflammatory response. It is expressed on activated microvascular endothelial cells in response to signals arising from immune responses in infection, graft rejection, tumor recognition and killing. The complementary binding ligand for VCAM-1 is VLA-4/CD49d. In addition to VCAM-1, VLA-4 also recognizes the extracellular matrix molecule fibronectin. This pairing of VCAM-1 and VLA-4 is able to provide a second signal (i.e., non-antigen specific) for T cell stimulation, such as that seen in transplantation. The monoclonal antibody MK-2 has been used in transplant studies to suppress cardiac rejection and induce long-term cardiac graft survival. In addition to inflammatory responses, VCAM-1 has a significant role in hemopoiesis through its ability to retain lymphocyte and myeloid precursors on stromal cells in the marrow and lymphoid organs. CD106/VCAM-1 exists as an integral membrane protein.

Gene ID: 22329

UniProt: P29533

Pathways: Carbohydrate Homeostasis