CD19 antibody

Catalog No. ABIN135152

The Rat Monoclonal anti-CD19 antibody is suitable to detect CD19 in samples from Mouse. It has been validated for FACS, IP, IHC (fro) and Sep.

Quick Overview for CD19 antibody (ABIN135152)

Target: CD19 (CD19 Molecule (CD19))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD19 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Separation (Sep)

Clone: 6D5

Product Details anti-CD19 Antibody

Purpose: Rat Anti-Mouse CD19-LE/AF

Specificity: Mouse CD19, Mr 95 kDa

Characteristics: Low Endotoxin/Azide Free Anti-Mouse CD19 antibody for use in flow cytometry, immunohistochemistry, immunoprecipitation, and separation assays.

Endotoxin Level: Low Endotoxin

Immunogen: Mouse CD19-expressing K562 human erythroleukemia cells

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Phosphate buffered saline, pH 7.4

Preservative: Azide free

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: 2-8°C

Target Details for CD19

Target: CD19 (CD19 Molecule (CD19))

Alternative Name: CD19

Background: CD19 is a monomeric transmembrane glycoprotein expressed at relatively constant levels throughout B cell development from early pro-B/pre-B cells (i.e. B220+/CD43+/HSA+) through fully differentiated B cell stages. Terminally differentiated plasma cells do not express CD19. In humans, the CD19 molecule on the surface of mature B cells associates with CD21 (CR-2) and CD81 (TAPA-1), and this multimolecular complex synergizes with surface immunoglobulin to provide signal transduction and promote cellular activation. All splenic and peritoneal IgM+ cells of both B-1 and B-2 lineages are CD19+, with B-1 cells expressing higher levels of CD19 than B-2 cells in these sites. Recent studies with CD19-deficient mice have suggested that this molecule may not be required for normal generation and maturation of B cells in the bone marrow.

Gene ID: 12478

UniProt: P25918

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway