FCER2 antibody

Catalog No. ABIN135164

The Rat Monoclonal anti-FCER2 antibody has been validated for FACS. It is suitable to detect FCER2 in samples from Mouse.

Quick Overview for FCER2 antibody (ABIN135164)

Target: FCER2 (Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This FCER2 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: 2G8

Product Details anti-FCER2 Antibody

Specificity: Mouse CD23, Mr 45-49 kDa

Characteristics: Rat Anti-Mouse CD23-UNLB

Purification: Purified

Immunogen: FcR isolated from B cell lymphoma cell line M12.4.5

Isotype: IgG2a

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , ELISA - Reported in literature
• Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC and BIOT conjugates 1 g/106 cells PE, APC, and PE/CY7 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

cell sorting

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Preservative: Without preservative

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for FCER2

Target: FCER2 (Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2))

Alternative Name: CD23

Background: The CD23 antigen is the low affinity IgE Fc receptor, which is a 49 kDa protein with 38 and 28 kDa fragments. It is expressed on most mature, conventional B cells (but not on peritoneal CD5+ B cells), and can also be found on the surface of T cells, macrophages and platelets. It is distinct from the high affinity IgE receptors found on basophils and mast cells, which mediate allergic reactions. The low affinity receptors are thought to play a role in isotype specific immunoregulation. The regulation of CD23 surface expression appears to be integral with the complex IgE system, which involves interactions of cells, cytokines, antibodies and regulatory factors. CD23 has been described as a membrane bound cytokine, in that the soluble cleavage products of CD23 are themselves able to act as cytokines in vitro.

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process