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- Target See all CD28 Antibodies
- CD28
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Reactivity
- Mouse
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Host
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Hamster
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Clonality
- Monoclonal
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Conjugate
- This CD28 antibody is un-conjugated
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Application
- Flow Cytometry (FACS)
- Specificity
- Mouse CD28, Mr 40 kDa
- Characteristics
- Hamster Anti-Mouse CD28-LE/AF
- Endotoxin Level
- Low endotoxin
- Immunogen
- C57BL/6N mouse T cell lymphoma EL-4 cell line
- Clone
- 37-51
- Isotype
- IgG
- Top Product
- Discover our top product CD28 Primary Antibody
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anti-CD28 (CD28) (AA 1-120) antibody
CD28 Reactivity: Human, Mouse, Rat FACS, WB, ELISA, IHC (fro), IF (p), IHC (p), IF (cc) Host: Rabbit Polyclonal unconjugated
anti-CD28 (CD28) antibodyCD28 Reactivity: Human, Non-Human Primate FACS, WB, IP, IHC (fro), ICC Host: Mouse Monoclonal CD28-2 unconjugated
anti-CD28 (CD28) antibody (FITC)CD28 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal CD28-2 FITC
anti-CD28 (CD28) (AA 19-152) antibodyCD28 Reactivity: Human FACS, ELISA Host: Rabbit Monoclonal DM61 unconjugated Recombinant Antibody
anti-CD28 (CD28) antibodyCD28 Reactivity: Human, Non-Human Primate FACS, WB, IP, Func, IHC (fro), ICC Host: Mouse Monoclonal CD28-2 unconjugated
anti-CD28 (CD28) antibodyCD28 Reactivity: Rat FACS, IP Host: Mouse Monoclonal JJ319 unconjugated
anti-CD28 (CD28) (C-Term) antibodyCD28 Reactivity: Human, Mouse, Rat WB, IHC Host: Rabbit Polyclonal unconjugated
anti-CD28 (CD28) antibody (PE)CD28 Reactivity: Rat FACS Host: Mouse Monoclonal JJ319 PE
anti-CD28 (CD28) (Internal Region) antibodyVerified CD28 Reactivity: Human WB, ELISA, IHC Host: Goat Polyclonal unconjugated
anti-CD28 (CD28) antibody (APC)CD28 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal CD28-2 APC
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- Application Notes
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- Applications: FC - Quality tested , IP - Reported in literature , Costim - Reported in literature , Stim - Reported in literature , Activ - Reported in literature , Block - Reported in literature
- Working Dilutions: Flow Cytometry FITC and BIOT conjugates 2 g/106 cells PE, APC, and SPRD conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
- Comment
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In vitro costimulation of T cells and NK cells
- Sample Volume
- 1 mL
- Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- 0.5 mg of purified immunoglobulin in 1.0 mL of PBS
- Preservative
- Azide free
- Handling Advice
- Each reagent is stable for the period shown on the bottle label if stored as directed.
- Storage
- -20 °C
- Storage Comment
- Aliquot and freeze the low endotoxin, azide-free product at -20 °C immediately upon receipt.
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- Target
- CD28
- Alternative Name
- CD28 (CD28 Products)
- Synonyms
- Tp44 antibody, CD28RNA antibody, cd28 antibody, CD28 molecule antibody, CD28 antigen antibody, Cd28 molecule antibody, CD28 molecule L homeolog antibody, CD28 antibody, Cd28 antibody, cd28.L antibody
- Background
- CD28 is a type I disulfide-linked homodimer that is constitutively expressed on most thymocytes, at low density on nearly all CD4+ and CD8+ peripheral T lymphocytes, and at very low levels on NK cells. Its expression is upregulated upon T-cell activation. CD28 is a ligand for CD80/B7-1 and CD86/B7-2 on B cells and other antigen presenting cells, and plays an important role in the interaction between T cells and B cells. CD28 is a co-stimulatory receptor involved in many, but not all, T-cell independent immune responses.
- Pathways
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
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