CD28 antibody

Catalog No. ABIN135188

The Hamster Monoclonal anti-CD28 antibody is suitable to detect CD28 in samples from Mouse. It has been validated for FACS, IP, St and CoSt.

Quick Overview for CD28 antibody (ABIN135188)

Target: CD28

Reactivity: Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This CD28 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Stimulation (St), Co-Stimulation (CoSt)

Clone: PV-1

Product Details anti-CD28 Antibody

Purpose: Hamster Anti-Mouse CD28-LE/AF

Specificity: Mouse CD28, Mr 40 kDa

Characteristics: Low Endotoxin/Azide Free Anti-Mouse CD28 antibody for use in flow cytometry, immunoprecipitation, costimulation, and stimulation assays.

Endotoxin Level: Low Endotoxin

Immunogen: C57BL/6N mouse T cell lymphoma EL4 cell line

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Phosphate buffered saline, pH 7.4

Preservative: Azide free

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: 2-8°C

Target Details for CD28

Target: CD28

Alternative Name: CD28

Background: CD28 is a type I disulfide-linked homodimer that is constitutively expressed on most thymocytes, at low density on nearly all CD4+ and CD8+ peripheral T lymphocytes, and at very low levels on NK cells. Its expression is upregulated upon T-cell activation. CD28 is a ligand for CD80/B7-1 and CD86/B7-2 on B cells and other antigen presenting cells, and plays an important role in the interaction between T cells and B cells. CD28 is a co-stimulatory receptor involved in many, but not all, T-cell independent immune responses.

Gene ID: 12487

UniProt: P31041

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response