L-Selectin antibody

Catalog No. ABIN135253

This Rat Monoclonal antibody specifically detects L-Selectin in FACS, IP, IHC (fro), BR and CyTox. It exhibits reactivity toward Mouse.

Quick Overview for L-Selectin antibody (ABIN135253)

Target: L-Selectin (SELL) (Selectin L (SELL))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This L-Selectin antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Reagent (BR), Cytotoxicity Test (CyTox)

Clone: MEL-14

Product Details anti-L-Selectin Antibody

Purpose: Rat Anti-Mouse CD62L-LE/AF

Specificity: Mouse CD62L, Mr 74 kDa on lymphocytes and Mr 95 kDa on neutrophils

Characteristics: Low Endotoxin/Azide Free Anti-Mouse CD62L antibody for use in flow cytometry, immunohistochemistry, immunoprecipitation, blocking, and cytotoxicity assays.

Endotoxin Level: Low Endotoxin

Immunogen: C3H/eb cloned mouse B cell lymphoma 38C-13

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Phosphate buffered saline, pH 7.4

Preservative: Azide free

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: 2-8°C

Target Details for L-Selectin

Target: L-Selectin (SELL) (Selectin L (SELL))

Alternative Name: CD62L

Background: CD62L, also known as L-selectin, is a type I transmembrane glycoprotein with lectin-like and Epidermal Growth Factor-like domains. It binds to sialylated oligosaccharide determinants on high endothelial venules (HEV) in peripheral lymph nodes. In the mouse, CD62L is expressed on most thymocytes and on peripheral leukocytes, including B and T lymphocytes, neutrophils, monocytes and eosinophils. This member of the selectin family of cell adhesion molecules appears to be required for lymphocyte homing to peripheral lymph nodes and for leukocyte extravasation at sites of inflammation. Expression of CD62L on lymphocytes and neutrophils is rapidly down-regulated upon cell activation and the level of CD62L expression, along with other markers, distinguishes naive T cells from effector/memory T cells.

Gene ID: 20343

UniProt: P18337