CD69 antibody

Catalog No. ABIN135258

This anti-CD69 antibody is a Hamster Monoclonal antibody detecting CD69 in FACS. Suitable for Mouse.

Quick Overview for CD69 antibody (ABIN135258)

Target: CD69

Reactivity: Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This CD69 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: H1-2F3

Product Details anti-CD69 Antibody

Specificity: Mouse CD69, Mr 85 kDa (unreduced)

Characteristics: Hamster Anti-Mouse CD69-LE/AF

Purification: Ion exchange

Endotoxin Level: Low endotoxin

Immunogen: Mouse dendritic epidermal cell line Y245

Isotype: IgG

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature , Costim - Reported in literature , Activ - Reported in literature
• Working Dilutions: Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE, APC, SPRD, PE/CY7, AF647, and PACBLU conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

In vitro T cell and NK-cell activation

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg of purified immunoglobulin in 1.0 mL of PBS

Preservative: Azide free

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: -20 °C

Storage Comment: Aliquot and freeze the low endotoxin, azide-free product at -20 °C immediately upon receipt.

Target Details for CD69

Target: CD69

Alternative Name: CD69

Background: CD69, also known as very early activation (VEA) antigen, is a disulfide-linked transmembrane homodimer whose differentially glycosylated subunits range from 35-39 kDa. It is a C-type lectin, most closely related to the NKR-P1 and Ly-49 NK cell-activation molecules. CD69 is widely expressed on hematopoietic cells, including lymphocytes, neutrophils and eosinophils. Although not detectable on resting lymphocytes, its expression is rapidly (within 2 hours) upregulated upon activation of T, B and NK cells, and neutrophils.4 Constitutive expression of CD69 on subsets of thymocytes suggests that it may be involved in regulation of developmental events in addition to its role in activation of a variety of hematopoietic cells.