CD86 antibody

Catalog No. ABIN135277

This Rat Monoclonal antibody specifically detects CD86 in FACS, IP, IHC (fro) and BR. It exhibits reactivity toward Mouse.

Quick Overview for CD86 antibody (ABIN135277)

Target: CD86

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD86 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Reagent (BR)

Clone: GL1

Product Details anti-CD86 Antibody

Purpose: Rat Anti-Mouse CD86-LE/AF

Specificity: Mouse CD86, Mr 80 kDa

Characteristics: Low Endotoxin/Azide Free Anti-Mouse CD86 antibody for use in flow cytometry, immunohistochemistry, immunoprecipitation, and blocking assays.

Endotoxin Level: Low Endotoxin

Immunogen: LPS-activated CBA/Ca mouse spleen B cells

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Phosphate buffered saline, pH 7.4

Preservative: Azide free

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: 2-8°C

Target Details for CD86

Target: CD86

Alternative Name: CD86

Background: CD86, also known as B7-2, is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B-cell specific stimuli with peak expression at 18-42 hours after stimulation. CD86, along with CD80/B7-1, is an important accessory molecule in T cell costimulation via its interaction with CD28 and CD152/CTLA-4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response.

Gene ID: 12524

UniProt: P42082

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation