Ly6C antibody (Biotin)

Catalog No. ABIN135295

This Rat Monoclonal antibody specifically detects Ly6C in FACS. It exhibits reactivity toward Mouse.

Quick Overview for Ly6C antibody (Biotin) (ABIN135295)

Target: Ly6C (Ly-6C) (Ly-6C)

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Ly6C antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Clone: HK1-4

Product Details anti-Ly6C Antibody (Biotin)

Specificity: Mouse Ly-6C, Mr 14-17 kDa

Characteristics: Rat Anti-Mouse Ly-6C-BIOT

Immunogen: L3 cloned CTL cells

Isotype: IgG2c

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , Activ - Reported in literature
• Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC, BIOT, and AF488 conjugates 1 g/106 cells PACBLU conjugate 0.3 g/106 cells PE conjugate 0.2 g/106 cells AF647 and AF700 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

In vitro activation of T lymphocytes

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL of PBS/Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for Ly6C

Target: Ly6C (Ly-6C) (Ly-6C)

Alternative Name: Ly-6C

Background: Ly-6C is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T-cell subsets. Mice with the Ly-6.2 allotype (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have subsets of CD4+Ly-6C+ and CD8+Ly-6C+ cells, while Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+Ly-6C+ lymphocytes. Ly-6C may play a role in the development and maturation of lymphocytes.