GRP78 antibody

Catalog No. ABIN135301

This Rat Monoclonal antibody specifically detects GRP78 in WB. It exhibits reactivity toward Mouse.

Quick Overview for GRP78 antibody (ABIN135301)

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This GRP78 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 76-E6

Product Details anti-GRP78 Antibody

Specificity: Mouse BiP, Mr 78 kDa

Characteristics: Rat Anti-Mouse BiP-UNLB

Purification: Purified

Immunogen: Mouse BiP: chain complexes

Isotype: IgG2a

Application Details

Application Notes:

• Applications: WB - Quality tested , IP - Reported in literature , ELISA - Reported in literature
• Working Dilutions: Immunoblotting Purified (UNLB) antibody 5 g/mL BIOT conjugate 5 g/mL

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Preservative: Without preservative

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for GRP78

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Alternative Name: BiP

Background: The immunoglobulin heavy chain binding protein BiP (Binding Protein) is a member of the hsp70 family of heat shock proteins, and is identical to the glucose regulated protein grp78.2 While BiP was originally described for its function in B cells, it is now known to be distributed in a variety of tissues, if not ubiquitous. The highly conserved hsp 70 proteins have an essential physiological role in stress responses and as molecular chaperones, which are responsible for a variety of functions such as protein transport, prevention of protein toxicity and direction of protein folding.1-5 With regard to its immunological role, BiP is a component of the endoplasmic reticulum and binds free intracellular heavy chains in nonsecreting pre-B cell lines (+,L-) or incompletely assembled Ig precursors in H+L+ secreting hybridomas and myelomas. In the absence of light chain synthesis, heavy chains remain associated with BiP and are not secreted. BiP is an ATP binding protein and the dissociation of the BiP-heavy chain complex is probably driven by the ATPase activity attributed to BiP.7

Pathways: Thyroid Hormone Synthesis, ER-Nucleus Signaling