IL2 Receptor beta antibody (FITC)

Catalog No. ABIN135409

This Rat Monoclonal antibody specifically detects IL2 Receptor beta in FACS. It exhibits reactivity toward Mouse.

Quick Overview for IL2 Receptor beta antibody (FITC) (ABIN135409)

Target: IL2 Receptor beta (IL2RB) (Interleukin 2 Receptor, beta (IL2RB))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL2 Receptor beta antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: 5H4

Product Details anti-IL2 Receptor beta Antibody (FITC)

Specificity: Mouse CD122, 90-100 kDa

Characteristics: Rat Anti-Mouse CD122-FITC

Immunogen: Rat myeloma YB2/0 transfected with truncated IL-2Rbeta cDNA (YB2/0-mbetat-28)

Isotype: IgG2a

Application Details

Application Notes:

• Applications: FC - Quality tested , IP - Reported in literature
• Working Dilutions: Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE and APC conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL of PBS/Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for IL2 Receptor beta

Target: IL2 Receptor beta (IL2RB) (Interleukin 2 Receptor, beta (IL2RB))

Alternative Name: CD122

Background: The IL-2 receptor is a complex of three distinct polypeptide chains: (i) the chain which binds IL-2 with low affinity, (ii) the chain that binds IL-2 with high affinity, and (ii) the common chain (C) that does not bind IL-2. The high affinity receptor complex is an α/β/γ heterotrimer with a kDa of 1.3 x 10-11 M.2 In mouse spleen, CD122 is expresed on 30 % of CD8+ cells and all NK cells, but < 1 % of B cells and CD4+ T lymphocytes. In the thymus, its expression is confined to CD4-CD8+ single positive and CD4-CD8- double negative cells. Cytoplasmic regions of the IL-2R chain are involved in IL-2-mediated cellular signaling and, via the interaction of IL-2 and its receptor complex, may be involved in the generation and differentiation of T lymphocytes.

Pathways: JAK-STAT Signaling, Growth Factor Binding