CD86 antibody

Catalog No. ABIN135433

This Rat Monoclonal antibody specifically detects CD86 in FACS, IP, ELISA, IHC (fro), BR, Sep and CMCD. It exhibits reactivity toward Mouse.

Quick Overview for CD86 antibody (ABIN135433)

Target: CD86

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD86 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Reagent (BR), Separation (Sep), Complement Mediated Cell Depletion (CMCD)

Clone: 2D10

Product Details anti-CD86 Antibody

Purpose: Rat Anti-Mouse CD86-LE/AF

Specificity: Mouse CD86, Mr 80 kDa

Characteristics: Low Endotoxin/Azide Free Anti-Mouse CD86 antibody for use in flow cytometry, immunohistochemistry, immunoprecipitation, ELISA, separation, CMCD, and blocking assays.

Endotoxin Level: Low Endotoxin

Immunogen: Mouse B cell lymphoma cell line 5C2

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Phosphate buffered saline, pH 7.4

Preservative: Azide free

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: 2-8°C

Target Details for CD86

Target: CD86

Alternative Name: CD86

Background: CD86/B7-2 is an 80 kDa type I integral membrane protein and a member of the immunoglobulin superfamily of cell surface receptors. Along with CD80/B7-1, CD86 participates in T cell costimulation via interaction with CD28 and CD152/CTLA-4. It is expressed at very low density in normal tissues but is rapidly upregulated on stimulated B cells, monocytes and dendritic cells. Since CD86/B7-2 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response.

Gene ID: 12524

UniProt: P42082

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation