ELK1 antibody (pThr417)

Catalog No. ABIN1355831

The Rabbit Polyclonal anti-ELK1 antibody has been validated for IHC and IF. It is suitable to detect ELK1 in samples from Human, Mouse and Rat.

Quick Overview for ELK1 antibody (pThr417) (ABIN1355831)

Target: ELK1 (ELK1, Member of ETS Oncogene Family (ELK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ELK1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-ELK1 Antibody

Binding Specificity: pThr417

Specificity: The antibody detects endogenous level of Elk1 only when phosphorylated at threonine 417.

Immunogen: Peptide sequence around phosphorylation site of pThr417 (L-S-T (p) -P-V) derived from Human Elk1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Application Details

Application Notes: Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C

Target Details for ELK1

Target: ELK1 (ELK1, Member of ETS Oncogene Family (ELK1))

Alternative Name: Elk1

Background:  Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.

Molecular Weight: 47 kDa

NCBI Accession: NM_001257, NP_001244

UniProt: P19419

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling