MCAM antibody (Biotin)

Catalog No. ABIN135588

This anti-MCAM antibody is a Mouse Monoclonal antibody detecting MCAM in FACS. Suitable for Chicken.

Quick Overview for MCAM antibody (Biotin) (ABIN135588)

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Reactivity: Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This MCAM antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Clone: C264

Product Details anti-MCAM Antibody (Biotin)

Specificity: Chicken/Turkey MCAM, Mr 98 kDa

Characteristics: Mouse Anti-Chicken MCAM-BIOT

Purification: The antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Mixture of E15 and E16 thymocytes from H.B15, H.B19, and H.B21 chicken embryos

Isotype: IgG2b

Application Details

Application Notes:

• Applications: FC - Quality tested , ICC - Reported in literature , IP - Reported in literature
• Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC and BIOT conjugates 1 g/106 cells PE conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL of PBS/Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for MCAM

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Alternative Name: MCAM

Background: Chicken MCAM, a member of the immunoglobulin superfamily of cell surface receptors, consists of the V-V-C2-C2-C2 Ig domains. It is a single chain glycoprotein with three mRNA splice variants. One has a short cytoplasmic tail, another has a long tail, and the third seems to lack transmembrane and cytoplasmic regions. It is highly homologous to gicerin, a molecule involved in neurite outgrowth and Wilm's kidney tumor progression in the chicken, and to the human melanoma progression molecule MUC18. Chicken MCAM is expressed by c-kit+ hematopoietic progenitor cells in embryonic bone marrow, thymocytes, and capillary endothelial cells. MCAM-expressing cells sorted from bone marrow are enriched in progenitors capable of differentiating into T cells when transferred into the appropriate thymic microenvironment. MCAM has been proposed to be involved in cellular adhesion and homing processes.