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Mouse anti-Human IgG1 (Hinge Region) Antibody (Biotin)

ELISA Monoclonal 4E3 IgG1 Biotin
Catalog No. ABIN135626

Quick Overview for Mouse anti-Human IgG1 (Hinge Region) Antibody (Biotin) (ABIN135626)

Target

IgG1

Clonality

  • 95
  • 63
Monoclonal

Conjugate

  • 53
  • 20
  • 20
  • 18
  • 11
  • 8
  • 7
  • 4
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Biotin

Application

  • 88
  • 66
  • 52
  • 51
  • 30
  • 25
  • 19
  • 16
  • 14
  • 13
  • 11
  • 10
  • 9
  • 8
  • 4
  • 3
  • 2
  • 2
  • 2
ELISA

Clone

4E3

Isotype

IgG1
  • Binding Specificity

    • 41
    • 25
    • 9
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Hinge Region

    Reactivity

    • 97
    • 27
    • 24
    • 9
    • 7
    • 5
    • 4
    • 3
    • 3
    • 2
    • 1
    Human

    Host

    • 60
    • 45
    • 42
    • 15
    • 10
    • 6
    • 1
    Mouse

    Specificity

    Reacts with the hinge region of the heavy chain of human IgG1 as demonstrated by ELISA

    Characteristics

    Mouse Anti-Human IgG1 Hinge-BIOT

    Purification

    Protein A affinity column

    Immunogen

    Unknown
  • Application Notes

    • Applications: ELISA - Quality tested , FLISA - Quality tested FC - Reported in literature , IHC-PS - Reported in literature , WB - Reported in literature , Multiplex - Reported in literature , Sep - Reported in literature , Purification - Reported in literature , Note - For direct immunostaining of IgG, positive cells in flow cytometry applications, clone HP, (SB Cat. No. ,) is recommended
    • Working Dilutions: ELISA Purified (UNLB) antibody ≤ 5 g/mL AP conjugate 1:1,000 - 1:2,000 HRP conjugate 1:2,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:10,000 FLISA FITC, AF488, and AF555 conjugates 1:200 - 1:400 PE and AF647 conjugates ≤ 1 g/mL

    Restrictions

    For Research Use only
  • Concentration

    0.5 mg/mL

    Buffer

    0.5 mg in 1.0 mL of PBS/Sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Dilute only prior to immediate use
    Each reagent is stable for the period shown on the bottle label if stored as directed.

    Storage

    4 °C

    Storage Comment

    Store at 2-8°C
  • Target

    IgG1

    Target Type

    Antibody
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