P-Selectin antibody

Catalog No. ABIN135713

The Mouse Monoclonal anti-P-Selectin antibody has been validated for FACS. It is suitable to detect P-Selectin in samples from Human.

Quick Overview for P-Selectin antibody (ABIN135713)

Target: P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This P-Selectin antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: 1-2B6

Product Details anti-P-Selectin Antibody

Specificity: Human CD62E & CD62P/Porcine CD62E, Mr 97 kDa/140 kDa

Characteristics: Mouse Anti-Human CD62E/CD62P-UNLB

Purification: Purified

Immunogen: TNF stimulated human endothelial cells

Isotype: IgG1

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , ICC - Reported in literature , IP - Reported in literature , WB-NR - Reported in literature , ELISA - Reported in literature , Block - Reported in literature , Adhesion - Reported in literature
• Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC, BIOT, PE, APC, SPRD, and AF700 conjugates 10 L/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

Inhibition of activity

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 0.1 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Preservative: Without preservative

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for P-Selectin

Target: P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

Alternative Name: CD62E/CD62P

Background: CD62E represents the 100-110 kDa type I transmembrane glycoprotein E-selectin, also known as endothelial-leukocyte adhesion molecule-1 (ELAM-1). It is expressed on cytokine-stimulated endothelium, weakly on unactivated endothelium, and is absent on leukocytes. Like other selectins, CD62E is an adhesion molecule that contributes to the initial attachment and rolling of leukocytes on endothelial surfaces, a prerequisite for leukocyte extravasation into tissues.