P-Selectin antibody (PE)

Catalog No. ABIN135715

This Mouse Monoclonal antibody specifically detects P-Selectin in FACS. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for P-Selectin antibody (PE) (ABIN135715)

Target: P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This P-Selectin antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: 1-2B6

Product Details anti-P-Selectin Antibody (PE)

Specificity: Human CD62E & CD62P/Porcine CD62E, Mr 97 kDa/140 kDa

Characteristics: Mouse Anti-Human CD62E/CD62P-PE

Purification: Ion exchange.

Immunogen: TNF stimulated human endothelial cells

Isotype: IgG1

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , ICC - Reported in literature , IP - Reported in literature , WB-NR - Reported in literature , ELISA - Reported in literature , Block - Reported in literature , Adhesion - Reported in literature
• Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC, BIOT, PE, APC, SPRD, and AF700 conjugates 10 L/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

Inhibition of activity

Restrictions: For Research Use only

Handling

Buffer: 100 tests in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References for anti-P-Selectin antibody (PE) (ABIN135715)

Fink, Schwarz, Feldbra: "Severe endothelial injury and subsequent repair in patients after successful cardiopulmonary resuscitation." in: Critical care, Vol. 14, Issue 3, pp. R104, (2010) (PubMed).

Target Details for P-Selectin

Target: P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

Alternative Name: CD62E/CD62P

Background: CD62E represents the 100-110 kDa type I transmembrane glycoprotein E-selectin, also known as endothelial-leukocyte adhesion molecule-1 (ELAM-1). It is expressed on cytokine-stimulated endothelium, weakly on unactivated endothelium, and is absent on leukocytes. Like other selectins, CD62E is an adhesion molecule that contributes to the initial attachment and rolling of leukocytes on endothelial surfaces, a prerequisite for leukocyte extravasation into tissues.