CD35 antibody (PE)

Catalog No. ABIN135767

The Mouse Monoclonal anti-CD35 antibody has been validated for FACS. It is suitable to detect CD35 in samples from Human.

Quick Overview for CD35 antibody (PE) (ABIN135767)

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD35 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: E11

Product Details anti-CD35 Antibody (PE)

Specificity: Human/Rhesus/Cynomolgus/Chimpanzee/Baboon CD35, Mr 160, 190, 220, 250 kDa

Characteristics: Mouse Anti-Human CD35-PE

Immunogen: Acute monocytic leukemia cells and normal blood monocytes

Isotype: IgG1

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , IP - Reported in literature , WB - Reported in literature , ELISA - Reported in literature
• Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC and PE conjugate 10 L/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Restrictions: For Research Use only

Handling

Buffer: 100 tests in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for CD35

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Alternative Name: CD35

Background: The CD35 antigen is composed of four different allotypes having different molecular weights (A (or F) - 160 kDa, B (or S) - 190 kDa, C - 220 kDa, and D - 250 kDa). It is the receptor for C3b and is found on B cells, a subset of T cells, monocytes, neutrophils, follicular dendritic cells, erythrocytes and kidney glomelular podocytes. CD35 mediates neutrophil and monocyte phagocytosis of particles coated with C3b and/or C4b. This interaction also enhances phagocytosis mediated by the Fc receptors.

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity