FAS antibody

Catalog No. ABIN135818

The Mouse Monoclonal anti-FAS antibody has been validated for FACS. It is suitable to detect FAS in samples from Human.

Quick Overview for FAS antibody (ABIN135818)

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FAS antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: DX3

Product Details anti-FAS Antibody

Specificity: Human/Rat CD95, Mr 45 kDa

Characteristics: Mouse Anti-Human CD95-UNLB

Purification: Purified

Immunogen: Human CD95 transfected L cells

Isotype: IgG2a

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-PS - Reported in literature , ELISA - Reported in literature
• Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC, BIOT, PE, APC and SPRD conjugates 10 L/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

in vivo induction of apoptosis

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 0.1 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Preservative: Without preservative

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for FAS

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Alternative Name: CD95

Background: CD95, also known as Fas and Apo-1, is a 40-50 kDa type I transmembrane glycoprotein and a member of the tumor necrosis factor receptor superfamily. It is expressed by activated lymphocytes, monocytes, neutrophils, fibroblasts and cell lines. Fas ligand binding to CD95 induces apoptosis in activated mature lymphocytes thereby playing a role in maintaining peripheral tolerance. Crosslinking of CD95 by the monoclonal antibodies DX2 and DX3 delivers an apoptotic signal to Fas-sensitive cells, indicating that these monoclonal antibodies recognize a functional epitope of CD95.

Pathways: p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity