Toxin A antibody

Catalog No. ABIN1381735

This Chicken egg antibody specifically detects in ELISA and WB. It exhibits reactivity toward Clostridium difficile.

Quick Overview for Toxin A antibody (ABIN1381735)

Target: Toxin A

Reactivity: Clostridium difficile

Host: Chicken egg

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details

Characteristics: Chickens were immunized with highly purified Toxin A purified from Clostridium difficile. After multiple immunizations in Freund's adjuvant, eggs were collected and the IgY purified. Anti-Clostridium difficile Toxin A was immunoaffinity purified using the immunogen immobilized on a solid support.

Purification: egg yolk-derived purified IgY, affinity purified

Application Details

Application Notes: This antibody is suitable for ELISA and Western Blotting procedures. By Western blot, a 1:4000 dilution of the IgY was an effective probe for 1 ug of Toxin A. By Direct ELISA, the half-point titre was 1:20,000 using a 2 ug/ml coating of Toxin A. Optimal working dilutions should be determined for your particular assay conditions.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.0 mg/ml

Buffer: Phosphate Buffered Saline (PBS), pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details

Target: Toxin A