Toxin B antibody
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- Target
- Toxin B
- Reactivity
- Clostridium difficile
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Host
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Chicken egg
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Conjugate
- Un-conjugated
- Application
- ELISA, Western Blotting (WB)
- Characteristics
- Chickens were immunized with highly purified Toxin B purified from Clostridium difficile. After multiple immunizations in Freund's adjuvant, eggs were collected and the IgY purified. Anti-Clostridium difficile Toxin B was immunoaffinity purified using the immunogen immobilized on a solid support.
- Purification
- egg yolk-derived purified IgY, affinity purified
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- Application Notes
- This antibody is suitable for ELISA and Western Blotting procedures. By Direct ELISA, the half-point titre was 1:20,000 using a 2 ug/ml coating of Toxin B. This antibody has also been successfully used in cytotoxicity and neutralization assays (please see embedded image). Optimal working dilutions should be determined for your particular assay conditions.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/ml
- Buffer
- Phosphate Buffered Saline (PBS), pH 7.2
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
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- Target
- Toxin B
- Synonyms
- CD0660 antibody, CD630DERM_06600 antibody, toxin B antibody, CD630DERM_RS03965 antibody
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