Toxin B antibody

Catalog No. ABIN1381738

This Chicken egg not_set antibody specifically detects Toxin B in ELISA and WB. It exhibits reactivity toward Clostridium difficile.

Quick Overview for Toxin B antibody (ABIN1381738)

Target: Toxin B

Reactivity: Clostridium difficile

Host: Chicken egg

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details

Characteristics: Chickens were immunized with highly purified Toxin B purified from Clostridium difficile. After multiple immunizations in Freund's adjuvant, eggs were collected and the IgY purified. Anti-Clostridium difficile Toxin B was immunoaffinity purified using the immunogen immobilized on a solid support.

Purification: egg yolk-derived purified IgY, affinity purified

Application Details

Application Notes: This antibody is suitable for ELISA and Western Blotting procedures. By Direct ELISA, the half-point titre was 1:20,000 using a 2 ug/ml coating of Toxin B. This antibody has also been successfully used in cytotoxicity and neutralization assays (please see embedded image). Optimal working dilutions should be determined for your particular assay conditions.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/ml

Buffer: Phosphate Buffered Saline (PBS), pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details

Target: Toxin B