Procollagen Type 2A (AA 1-100) antibody

Catalog No. ABIN1385152

Product Details

Target: Procollagen Type 2A

Binding Specificity: AA 1-100

Reactivity: Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Immunocytochemistry (ICC)

Cross-Reactivity: Zebrafish (Danio rerio)

Predicted Reactivity: Human,Dog,Cow,Sheep,Pig,Chicken,Rabbit,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Collagen II alpha 1

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References

Tarafder, Gulko, Sim, Yang, Cook, Lee: "Engineered Healing of Avascular Meniscus Tears by Stem Cell Recruitment." in: Scientific reports, Vol. 8, Issue 1, pp. 8150, (2018) (PubMed).

Target Details

Target: Procollagen Type 2A

Background:

Synonyms: Collagen II N-terminal propeptide, Collagen II alpha 1, COL2A1, COL2A1 protein, collagen, type II, alpha 1, collagen alpha-1II, type II collagen, alpha-1 type II collagen, alpha1 type II collagen, Col2a1, AOM, Cartilage collagen, COL11A3, Collagen alpha 1II chain precursor, Collagen II alpha 1 polypeptide, Collagen type II alpha 1 primary osteoarthritis spondyloepiphyseal dysplasia congenital, MGC131516, SEDC, Collagen alpha-1II chain, Alpha-1 type II collagen, CO2A1_HUMAN.

Background: Collagen type II is the most abundant protein of cartilage matrix. Two isoforms exist and collagen type IIA is involved in early development of cartilage in embryos. The collagen is produced from type II procollagen when two teloterminal propeptides are digested from the precursor and released into the circulation. Procollagen type II N-terminal propeptide is considered a marker of cartilage synthesis and the normal concentration in serum is approximately 30 ng/mL.