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EGF antibody (AA 1-30)

Name: EGF antibody (AA 1-30)
SKU: ABIN1385262
Price: 384.62 USD
Availability: InStock

This anti-EGF antibody is a Rabbit Polyclonal antibody detecting EGF in ELISA, IF (p), IF (cc) and IHC (fro). Suitable for Human.

Paraformaldehyde-fixed, paraffin embedded human kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with EGF Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary Goat Anti-Rabbit IgG, FITC conjugated-FITC)used at 1:200 dilution for 40 minutes at 37°C. (EGF antibody (AA 1-30))

IF (p)

1 image

Catalog No. ABIN1385262

Quick Overview for EGF antibody (AA 1-30) (ABIN1385262)

Target

See all EGF Antibodies

EGF (Epidermal Growth Factor (EGF))

Reactivity

Human

Host

Rabbit

Clonality

Polyclonal

Conjugate

This EGF antibody is un-conjugated

Application

ELISA, Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Discover our top product EGF Primary Antibody

Binding Specificity
45

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AA 1-30

Cross-Reactivity

Human

Purification

Purified by Protein A.

Immunogen

MAP conjugated synthetic peptide derived from human EGF

Isotype

IgG
anti-Epidermal Growth Factor (EGF) (AA 974-1026) antibody
EGF Reactivity: Rat WB, IHC, IP, ICC Host: Mouse Monoclonal C11 unconjugated

anti-Epidermal Growth Factor (EGF) (AA 690-720), (Center) antibody
EGF Reactivity: Human, Mouse WB, IHC (p) Host: Rabbit Polyclonal RB16555 unconjugated

anti-Epidermal Growth Factor (EGF) antibody
EGF Reactivity: Human WB, DB Host: Rabbit Polyclonal unconjugated

anti-Epidermal Growth Factor (EGF) antibody
EGF Reactivity: Human IHC, ELISA Host: Mouse Monoclonal 9D7F11 unconjugated

anti-Epidermal Growth Factor (EGF) (AA 609-751) antibody
EGF Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Epidermal Growth Factor (EGF) antibody
EGF Reactivity: Human ELISA, ICC Host: Mouse Monoclonal 4E11 unconjugated

anti-Epidermal Growth Factor (EGF) antibody
EGF Reactivity: Mouse WB, ELISA, Neut Host: Goat Polyclonal unconjugated

anti-Epidermal Growth Factor (EGF) (AA 974-1026) antibody
EGF Reactivity: Rat WB, IHC, ELISA Host: Rabbit Polyclonal unconjugated

anti-Epidermal Growth Factor (EGF) (AA 977-1029) antibody
EGF Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Epidermal Growth Factor (EGF) (AA 609-751) antibody
EGF Reactivity: Human WB, IHC, IP, ICC Host: Mouse Monoclonal D2 unconjugated

Application Notes

ELISA 1:500-1000
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
IEM 1:20-200
IGS 1:20-200
GICA 1:20-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Target

EGF (Epidermal Growth Factor (EGF))

Alternative Name

Egf

Background

Synonyms: URG, HOMG4, Pro-epidermal growth factor, EGF
Background: EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).

Gene ID

1950

UniProt

P01133

Pathways

NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation