UBE2H antibody (AA 101-183)

Catalog No. ABIN1385713

This Rabbit Polyclonal antibody specifically detects UBE2H in WB, ELISA, IF (cc), IF (p), IHC (fro) and IHC (p). It exhibits reactivity toward Human.

Quick Overview for UBE2H antibody (AA 101-183) (ABIN1385713)

Target: UBE2H (Ubiquitin-Conjugating Enzyme E2H (UBE2H))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2H antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-UBE2H Antibody

Binding Specificity: AA 101-183

Predicted Reactivity: Mouse,Rat,Dog,Cow,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Ube2H/UBCH2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for UBE2H

Target: UBE2H (Ubiquitin-Conjugating Enzyme E2H (UBE2H))

Alternative Name: Ube2H

Background:

Synonyms: E2 20K, Ubc H2, UBC8, UBCH 2, UBCH, UBCH2, Ube2h, UBE2H_HUMAN, Ubiquitin carrier protein H, Ubiquitin conjugating enzyme E2 H, Ubiquitin conjugating enzyme E2H UBC8 homolog yeast, Ubiquitin conjugating enzyme E2H, Ubiquitin protein ligase H, Ubiquitin-conjugating enzyme E2 H, Ubiquitin-conjugating enzyme E2-20K, Ubiquitin-protein ligase H.

Background: UBE2H, also known as UBC8, UBCH, UBCH2 or E2-20K, is a 183 amino acid protein involved in ubiquitin-mediated protein degradation. Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). One of several members of the ubiquitin-conjugating enzyme family, UBE2H functions as an E2 ubiquitin-conjugating enzyme that acts to catalyze the covalent attachment of ubiquitin residues to various proteins, including Histone H2A. UBE2H shares 100 % identity with its mouse counterpart and 98 % identity with its frog and zebrafish homologs, suggesting a conserved function between species. Multiple isoforms of UBE2H exist due to alternative splicing events.

Gene ID: 7328