UBE2H antibody

Catalog No. ABIN7231996

The Rabbit Polyclonal anti-UBE2H antibody is suitable to detect UBE2H in samples from Human and Mouse. It has been validated for WB and ELISA.

Quick Overview for UBE2H antibody (ABIN7231996)

Target: UBE2H (Ubiquitin-Conjugating Enzyme E2H (UBE2H))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2H antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-UBE2H Antibody

Purpose: Rabbit Anti-UBE2H Polyclonal Antibody

Specificity: The antibody detects endogenous levels of UBE2H protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human UBE2H protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for UBE2H

Target: UBE2H (Ubiquitin-Conjugating Enzyme E2H (UBE2H))

Alternative Name: UBE2H

Background: Ubiquitin-conjugating enzyme E2 H, E2 ubiquitin-conjugating enzyme H, UbcH2, Ubiquitin carrier protein H, Ubiquitin-conjugating enzyme E2-20K, Ubiquitin-protein ligase HUBE2H (Ubiquitin Conjugating Enzyme E2 H) is a Protein Coding gene. Diseases associated with UBE2H include Autistic Disorder. Among its related pathways are Protein ubiquitination and Class I MHC mediated antigen processing and presentation. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2H encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100 % identical to the mouse homolog and 98 % identical to the frog and zebrafish homologs. Three alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms.

Molecular Weight: 20kD

Gene ID: 7328

UniProt: P62256