OGT antibody (AA 951-1046)

Catalog No. ABIN1385759

The Rabbit Polyclonal anti-OGT antibody is suitable to detect OGT in samples from Rat. It has been validated for WB, ELISA, IHC (p), IHC (fro), IF (p) and IF (cc).

Quick Overview for OGT antibody (AA 951-1046) (ABIN1385759)

Target: OGT (O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OGT antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Product Details anti-OGT Antibody

Binding Specificity: AA 951-1046

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Cow

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human O-GlcNAc transferase

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for OGT

Target: OGT (O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT))

Alternative Name: O-GlcNAc transferase

Background:

Synonyms: HRNT1, MGC22921, O-GlcNAc transferase, O GlcNAc, O-Linked N-Acetylglucosamine Transferase, O GlcNAc transferase p110 subunit, O GlcNAc transferase subunit p110, O linked N acetylglucosamine GlcNAc transferase UDP N acetylglucosamine:polypeptide N acetylglucosaminyl transferase, O linked N acetylglucosamine transferase 110 kDa subunit, O-GlcNAc transferase subunit p110, O-linked N-acetylglucosamine transferase 110 kDa subunit, OGT, OGT1_HUMAN, UDP N acetylglucosamine peptide N acetylglucosaminyltransferase 110 kDa subunit, UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit, Uridinediphospho N acetylglucosamine:polypeptide beta N acetylglucosaminyl transferase.

Background: Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. O-GlcNAc transferase has been purified from rat liver. It exists as a heterotrimeric complex with two subunits of the same molecular mass and one shorter subunit. Both polypeptides are related, the short subunit band is either a proteolytic product of the polypeptide or the product of an alternative translation start site. O-GlcNAc transferase is expressed as multiple transcripts that are present in different amounts in various human tissues, with the highest levels of expression in pancreas. Immunofluorescence of human cells expressing rat O-GlcNAc transferase indicated that it is present in both the nucleus and cytosol. HeLa cells expressing O-GlcNAc transferase do not survive well during prolonged incubations, suggesting that this protein may be toxic to the cells.

Gene ID: 8473

UniProt: O15294

Pathways: Regulation of Carbohydrate Metabolic Process