POU4F2 antibody (AA 301-400)

Catalog No. ABIN1385914

This Rabbit Polyclonal antibody specifically detects POU4F2 in WB, ELISA, FACS, IF (cc), IF (p), IHC (fro) and IHC (p). It exhibits reactivity toward Mouse and Rat.

Quick Overview for POU4F2 antibody (AA 301-400) (ABIN1385914)

Target: POU4F2 (POU Domain, Class 4, Transcription Factor 2 (POU4F2))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POU4F2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-POU4F2 Antibody

Binding Specificity: AA 301-400

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Dog,Cow,Sheep,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human BRN3B/POU4F2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for POU4F2

Target: POU4F2 (POU Domain, Class 4, Transcription Factor 2 (POU4F2))

Alternative Name: BRN3B

Background:

Synonyms: BRN3B, class 4, transcription factor 2, Brain specic homeobox/POU domain protein 3B, Brain-3B, Brain-specic homeobox/POU domain protein 3B, Brn-3B, BRN3.2, Brn3b POU domain transcription factor, PO4F2_HUMAN, POU class 4 homeobox 2, POU domain, POU domain class 4 transcription factor 2, POU domain protein, POU4F2.

Background: The Brn family of transcription factors are found in a highly restricted subset of neurons and are critical to the early embryonic development of the central nervous system. Brn-1 and Brn-2 are class III POU (Pit-Oct-Unc) domain proteins, whereas Brn-3 is a class IV POU domain protein. Three Brn-3 proteins have been described and are designated Brn-3a, Brn-3b and Brn-3c. While Brn-3a and Brn-3c stimulate transcription, Brn-3b generally functions as a transcriptional repressor. However, Brn-3b, but not Brn-3a, has been shown to regulate the expression of the acetylcholine receptor. Interestingly, Brn-3a has two functional transactivating domains, one at the amino-terminus and one at the carboxy-terminus. Brn-2 is thought to be involved in smooth muscle cell development and differentiation.

Gene ID: 5458

UniProt: Q12837

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Sensory Perception of Sound