BPTF antibody (AA 2801-3046)

Catalog No. ABIN1386560

The Rabbit Polyclonal anti-BPTF antibody has been validated for WB, IF (cc), IF (p), ELISA, IHC (p), IHC (fro) and ICC. It is suitable to detect BPTF in samples from Mouse and Rat.

Quick Overview for BPTF antibody (AA 2801-3046) (ABIN1386560)

Target: BPTF (Bromodomain PHD Finger Transcription Factor (BPTF))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BPTF antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Product Details anti-BPTF Antibody

Binding Specificity: AA 2801-3046

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Dog,Cow,Sheep,Pig,Chicken,Rabbit,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human BPTF/FALZ

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for BPTF

Target: BPTF (Bromodomain PHD Finger Transcription Factor (BPTF))

Alternative Name: BPTF/FALZ

Background:

Synonyms: Bromodomain and PHD finger containing transcription factor, Bromodomain PHD finger transcription factor, FAC 1, BPTF / FALZ, fac1, FALZ, Fetal Alz 50 clone 1 protein, Fetal Alz 50 reactive clone 1, Fetal Alzheimer antigen, Nucleosome remodeling factor, Nucleosome remodeling factor subunit BPTF, Nucleosome remodeling factor, large subunit, NURF 301, NURF301.

Background: BPTF is a 2,907 amino acid protein encoded by the human gene BPTF. BPTF belongs to the PBTF family and contains one bromodomain, one DDT domain and two PHD-type zinc fingers. BPTF acts as a histone-binding component of NURF (nucleosome-remodeling factor). The NURF complex, which consists of SMARCA1, BPTF, RbAp46 and RbAp48, acts to catalyze ATP-dependent nucleosome sliding and facilitates transcription of chromatin. It specifically recognizes histone H3 tails trimethylated on 'Lys-4' (H3-K4Me3), which mark transcription start sites of virtually all active genes. BPTF may also help regulate transcription through direct binding to DNA or transcription factors.