BPTF antibody

Catalog No. ABIN968990

There are 2+ publications for this product available. The Mouse Monoclonal anti-BPTF antibody is suitable to detect BPTF in samples from Human. It has been validated for ELISA.

Quick Overview for BPTF antibody (ABIN968990)

Target: BPTF (Bromodomain PHD Finger Transcription Factor (BPTF))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BPTF antibody is un-conjugated

Application: ELISA

Clone: 2F10

Product Details anti-BPTF Antibody

Purpose: BPTF Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human BPTF expressed in E. Coli.

Isotype: IgG2b

Application Details

Application Notes: ELISA: 1/10000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-BPTF antibody (ABIN968990)

Landry, Sharov, Piao, Sharova, Xiao, Southon, Matta, Tessarollo, Zhang, Ko, Kuehn, Yamaguchi, Wu: "Essential role of chromatin remodeling protein Bptf in early mouse embryos and embryonic stem cells." in: PLoS genetics, Vol. 4, Issue 10, pp. e1000241, (2008) (PubMed).

Barbe, Lundberg, Oksvold, Stenius, Lewin, Björling, Asplund, Pontén, Brismar, Uhlén, Andersson-Svahn: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, (2008) (PubMed).

Target Details for BPTF

Target: BPTF (Bromodomain PHD Finger Transcription Factor (BPTF))

Alternative Name: BPTF

Background: BPTF (bromodomain and PHD domain transcription factor) is the largest subunit of the ATP-dependent chromatin-remodelling complex, NURF (nucleosome remodelling factor). NURF catalyses ATP-dependent nucleosome sliding and facilitates transcription. BPTF recognises histone H3 tails that are tri-methylated at K4, which marks the transcriptional start site of the vast majority of transcriptionally active genes. BPTF also exhibits some binding to H3 di-methylated at K4. BPTF plays a key role in the development of early mouse embryos, possibly through regulation of the Smad pathway of transcription factors. While BPTF is expressed in low levels in the adult brain and spinal cord, it is expressed in higher levels in the brain in neurodegenerative diseases. It is present in a subset of amyloid-containing plaques in the brains of patients suffering from Alzheimer's disease. Abundantly expressed in the fetal brain. Present throughout the gray and white matter of the developing spinal cord at 18-22 gestational weeks. Expressed at low levels in adult brain and spinal cord and reexpressed in neurodegenerative diseases (at protein level) .Tissue specificity: Ubiquitously expressed, with highest levels in testis. Present in kidney, liver and brain. In the brain, highest levels are found in motor cortex (at protein level).

Molecular Weight: 338 kDa

Gene ID: 2186

UniProt: Q12830