MARCH8 antibody

Catalog No. ABIN1387037

The Rabbit Polyclonal anti-MARCH8 antibody has been validated for WB, IF (p) and IHC (p). It is suitable to detect MARCH8 in samples from Human, Mouse and Rat.

Quick Overview for MARCH8 antibody (ABIN1387037)

Target: MARCH8 (Membrane-Associated Ring Finger (C3HC4) 8, E3 Ubiquitin Protein Ligase (MARCH8))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MARCH8 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MARCH8 Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human MARCH8

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for MARCH8

Target: MARCH8 (Membrane-Associated Ring Finger (C3HC4) 8, E3 Ubiquitin Protein Ligase (MARCH8))

Alternative Name: MARCH8

Background:

Synonyms: c MIR, c-MIR, Cellular modulator of immune recognition, E3 ubiquitin protein ligase MARCH8, E3 ubiquitin-protein ligase MARCH8, MARCH 8, MARCH VIII, MARCH-VIII, MARCH8, MARH8_HUMAN, Membrane associated RING CH protein VIII, Membrane associated ring finger C3HC4 8, Membrane associated RING finger protein 8, Membrane-associated RING finger protein 8, Membrane-associated RING-CH protein VIII, MIR, RING finger protein 178, RNF178.

Background: The RING-type zinc finger motif is present in a number of viral and eukaryotic proteins and is made of a conserved cysteine-rich domain that is able to bind two zinc atoms. Proteins that contain this conserved domain are generally involved in the ubiquitination pathway of protein degradation. MARCH8 (membrane-associated ring finger (C3HC4) 8), also known as MIR or RNF178 (RING finger protein 178), is a 291 amino acid multi-pass membrane protein that localizes to vesicle membranes and contains one RING-CH-type zinc finger. Expressed in a variety of tissues, including immature dendritic cells, MARCH8 functions as an E3 ubiquitin ligase that is thought to regulate immune responses by promoting the ubiquitination and subsequent degradation of target proteins, such as B7-2 and CD71.

Gene ID: 220972