SHARPIN antibody (AA 260-310)

Catalog No. ABIN1387105

This Rabbit Polyclonal antibody specifically detects SHARPIN in WB, IF (p) and IHC (p). It exhibits reactivity toward Human.

Quick Overview for SHARPIN antibody (AA 260-310) (ABIN1387105)

Target: SHARPIN (SHANK-Associated RH Domain Interacting Protein (SHARPIN))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SHARPIN antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SHARPIN Antibody

Binding Specificity: AA 260-310

Purpose: SHARPIN Polyclonal Antibody

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SHARPIN

Isotype: IgG

Application Details

Application Notes: WB(1:300-5000), IHC-P(1:200-400), IF(IHC-P)(1:50-200)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for SHARPIN

Target: SHARPIN (SHANK-Associated RH Domain Interacting Protein (SHARPIN))

Alternative Name: SHARPIN

Background:

Synonyms: SIPL1, Sharpin, Shank-associated RH domain-interacting protein, Shank-interacting protein-like 1, hSIPL1, PSEC0216

Background: Component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with FAM105B/otulin, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis.

Gene ID: 81858

UniProt: Q9H0F6