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CD19 antibody (AA 151-250)

Name: CD19 antibody (AA 151-250)
SKU: ABIN1387313
Price: 384.62 USD
Availability: InStock

The Rabbit Polyclonal anti-CD19 antibody has been validated for FACS, WB, ELISA and IHC (p). It is suitable to detect CD19 in samples from Human, Mouse and Rat.

Mouse splenocytes probed with Rabbit Anti-CD19 Polyclonal Antibody, PE-Cy5 Conjugated . (CD19 antibody (AA 151-250))

FACS

Antigen: 2 µg/100 µL Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; Secondary: HRP conjugated Rabbit Anti-Goat IgG at 1: 5000; TMB staining Read the data in Microplate Reader by 450nm. (CD19 antibody (AA 151-250))

ELISA

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-CD19 Polyclonal Antibody, Unconjugated (ABIN1387313) at 1:200 followed by conjugation to the secondary antibody and DAB staining. (CD19 antibody (AA 151-250))

IHC

Formalin-fixed and paraffin-embedded mouse intestine labeled with Anti-CD19 Polyclonal Antibody, Unconjugatedused at 1:200 dilution for 40 minutes at 37°C. (CD19 antibody (AA 151-250))

IF (p)

Mouse lung and liver lysates probed with CD19 Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C. (CD19 antibody (AA 151-250))

Paraformaldehyde-fixed, paraffin embedded fetal mouse brain tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with CD19 Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining (CD19 antibody (AA 151-250))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded mouse spleen tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with CD19 Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining (CD19 antibody (AA 151-250))

IHC (p)

7 images

Catalog No. ABIN1387313

Quick Overview for CD19 antibody (AA 151-250) (ABIN1387313)

Target

See all CD19 Antibodies

CD19 (CD19 Molecule (CD19))

Reactivity

Human, Mouse, Rat

Host

Rabbit

Clonality

Polyclonal

Conjugate

This CD19 antibody is un-conjugated

Application

Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Binding Specificity
25

21

16

15

14

12

10

7

7

4

4

4

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 151-250

Cross-Reactivity

Human, Mouse, Rat

Predicted Reactivity

Pig,Guinea Pig

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human CD19

Isotype

IgG
anti-CD19 Molecule (CD19) (AA 20-291) antibody
CD19 Reactivity: Human FACS, WB, IHC, IF, ELISA Host: Mouse Monoclonal 8G11B7 unconjugated

anti-CD19 Molecule (CD19) (C-Term) antibody
CD19 Reactivity: Human, Mouse, Rat, Monkey, Sheep, Pig, Cow, Guinea Pig, Rabbit, Dog, Avian, Cat, Chicken, Donkey, Goat, Hamster, Horse WB Host: Goat Polyclonal unconjugated

anti-CD19 Molecule (CD19) (AA 485-556) antibody
CD19 Reactivity: Human, Mouse, Rat, Sheep FACS, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

anti-CD19 Molecule (CD19) (pTyr531) antibody
CD19 Reactivity: Human, Mouse FACS, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

anti-CD19 Molecule (CD19) (AA 96-281) antibody
CD19 Reactivity: Human FACS, IHC, ELISA, Coat, StM Host: Mouse Monoclonal CD19-3116 unconjugated

anti-CD19 Molecule (CD19) antibody
CD19 Reactivity: Human FACS, WB, IF, ICC, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-CD19 Molecule (CD19) (AA 143-172) antibody
CD19 Reactivity: Human FACS, WB, IHC, IF, ELISA Host: Rabbit Polyclonal unconjugated

anti-CD19 Molecule (CD19) antibody
CD19 Reactivity: Human FACS, ICC Host: Mouse Monoclonal 4G7 unconjugated

anti-CD19 Molecule (CD19) antibody
CD19 Reactivity: Human FACS, IP Host: Mouse Monoclonal LT19 unconjugated

anti-CD19 Molecule (CD19) (AA 160-258) antibody
CD19 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

Application Notes

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Target

CD19 (CD19 Molecule (CD19))

Alternative Name

CD19

Background

Synonyms: B4, CVID3, B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, Differentiation antigen CD19, T-cell surface antigen Leu-12, CD19
Background: Assembles with the antigen receptor of B-lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.

Gene ID

930

UniProt

P15391

Pathways

Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway