COL2 antibody (AA 1401-1487)

Catalog No. ABIN1387355

There is 1 publication for this product available. The Rabbit Polyclonal anti-COL2 antibody is suitable to detect COL2 in samples from Human, Rat and Rabbit. It has been validated for WB, ELISA and IHC (p).

Quick Overview for COL2 antibody (AA 1401-1487) (ABIN1387355)

Target: COL2 (Collagen, Type II (COL2))

Reactivity: Human, Rat, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COL2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-COL2 Antibody

Binding Specificity: AA 1401-1487

Cross-Reactivity: Human, Rabbit, Rat

Predicted Reactivity: Mouse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Collagen II

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-COL2 antibody (ABIN1387355)

Yang, Lu, Guo: "Platelet-rich plasma protects rat chondrocytes from interleukin-1β-induced apoptosis." in: Molecular medicine reports, Vol. 14, Issue 5, pp. 4075-4082, (2016) (PubMed).

Target Details for COL2

Target: COL2 (Collagen, Type II (COL2))

Alternative Name: Collagen 2

Background:

Synonyms: Collagen II alpha 1, COL2A1, COL2A1 protein, collagen, type II, alpha 1, collagen alpha-1II, type II collagen, alpha-1 type II collagen, alpha1 type II collagen, Col2a1, AOM, Cartilage collagen, Chondrocalcin, COL11A3, Collagen alpha 1II chain precursor, Collagen type II alpha 1 primary osteoarthritis spondyloepiphyseal dysplasia congenital, MGC131516, SEDC.

Background: Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate with specificities to collagens. The development of type specific is dependent on NON DENATURED three dimensional epitopes. This may result in diminished reactivity of some with denatured collagen or formalin fixed, paraffin embedded tissues. Type II collagen is a fibrillar collagen found in cartilage and the vitreous humor of the eye. Collagen type II is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene.