DIAPH1 antibody (AA 41-150)

Catalog No. ABIN1387366

This Rabbit Polyclonal antibody specifically detects DIAPH1 in WB, ELISA, IHC (p), IF (cc), IF (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for DIAPH1 antibody (AA 41-150) (ABIN1387366)

Target: DIAPH1 (Diaphanous Homolog 1 (DIAPH1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DIAPH1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-DIAPH1 Antibody

Binding Specificity: AA 41-150

Predicted Reactivity: Human,Mouse,Dog,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DIAPH1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for DIAPH1

Target: DIAPH1 (Diaphanous Homolog 1 (DIAPH1))

Alternative Name: Diaph1

Background:

Synonyms: DIAPH1, FLJ25265, Deafness autosomal dominant 1, deafness, autosomal dominant 1, DFNA1, DIAP1, DIAP1_HUMAN, DIAPH1, diaphanous homolog 1, Diaphanous protein homolog 1, Diaphanous related formin 1, Diaphanous-related formin-1, DRF1, hDIA1, LFHL1, low frequency hearing loss 1, p140DIA, Protein diaphanous homolog 1.

Background: Acts in a Rho-dependent manner to recruit PFY1 to the membrane. Required for the assembly of F-actin structures, such as actin cables and stress fibers. Nucleates actin filaments. Binds to the barbed end of the actin filament and slows down actin polymerization and depolymerization. Required for cytokinesis, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Functions as a scaffold protein for MAPRE1 and APC to stabilize microtubules and promote cell migration (By similarity). Has neurite outgrowth promoting activity (By similarity). In hear cells, it may play a role in the regulation of actin polymerization in hair cells. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.

Gene ID: 1729

Pathways: Sensory Perception of Sound