ERN1 antibody (AA 252-260)

Catalog No. ABIN1387555

This Rabbit Polyclonal antibody specifically detects ERN1 in WB, ELISA, FACS, IF (cc), IF (p), ICC, IHC (p) and IHC (fro). It exhibits reactivity toward Human, Mouse, Rat and Pig and has been mentioned in 6+ publications.

Quick Overview for ERN1 antibody (AA 252-260) (ABIN1387555)

Target: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Reactivity: Human, Mouse, Rat, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ERN1 Antibody

Binding Specificity: AA 252-260

Cross-Reactivity: Human, Mouse, Pig, Rat

Predicted Reactivity: Dog,Cow,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human IRE1a

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-ERN1 antibody (ABIN1387555)

Chen, Wang, Chen, Wang, Gui, Dai, Fang, Zhang, Lu: "Influence of resveratrol on endoplasmic reticulum stress and expression of adipokines in adipose tissues/adipocytes induced by high-calorie diet or palmitic acid." in: Endocrine, Vol. 55, Issue 3, pp. 773-785, (2017) (PubMed).

He, Xu, Gou, Yuan, Liao, Chen, Fan, Xie, Deng, Zhang, Chen, Zhao: "CSFV Infection Up-Regulates the Unfolded Protein Response to Promote Its Replication." in: Frontiers in microbiology, Vol. 8, pp. 2129, (2017) (PubMed).

Jiang, Yun, Shi, Niu, Gao, Xie, Yu: "Downregulation of miR-384-5p attenuates rotenone-induced neurotoxicity in dopaminergic SH-SY5Y cells through inhibiting endoplasmic reticulum stress." in: American journal of physiology. Cell physiology, Vol. 310, Issue 9, pp. C755-63, (2016) (PubMed).

Xu, Su, Song, Shi, Fu, Hu, Xia, Song, Song: "Polychlorinated biphenyl quinone induces endoplasmic reticulum stress, unfolded protein response, and calcium release." in: Chemical research in toxicology, Vol. 28, Issue 6, pp. 1326-37, (2015) (PubMed).

Li, Han, Shi: "IRE1?-XBP1 Pathway Is Activated Upon Induction of Single-Prolonged Stress in Rat Neurons of the Medial Prefrontal Cortex." in: Journal of molecular neuroscience : MN, (2015) (PubMed).

Jiang, Ren, Jiang, Wang, Zhang, Yin, Wang, Qi, Wang, Feng: "Guanabenz delays the onset of disease symptoms, extends lifespan, improves motor performance and attenuates motor neuron loss in the SOD1 G93A mouse model of amyotrophic lateral sclerosis." in: Neuroscience, Vol. 277, pp. 132-8, (2014) (PubMed).

Target Details for ERN1

Target: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Alternative Name: IRE1a

Background:

Synonyms: IRE1, IRE1P, IRE1a, hIRE1p, Serine/threonine-protein kinase/endoribonuclease IRE1, Endoplasmic reticulum-to-nucleus signaling 1, Inositol-requiring protein 1, Ire1-alpha, ERN1

Background: Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.

Gene ID: 2081

UniProt: O75460

Pathways: ER-Nucleus Signaling, Unfolded Protein Response