DEPTOR antibody (AA 56-170)

Catalog No. ABIN1387597

This Rabbit Polyclonal antibody specifically detects DEPTOR in WB, IF (cc), IF (p), ELISA, IHC (p) and IHC (fro). It exhibits reactivity toward Human, Mouse and Rat and has been mentioned in 2+ publications.

Quick Overview for DEPTOR antibody (AA 56-170) (ABIN1387597)

Target: DEPTOR (DEP Domain Containing mTOR-Interacting Protein (DEPTOR))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DEPTOR antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-DEPTOR Antibody

Binding Specificity: AA 56-170

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DEPTOR/DEPDC6

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-DEPTOR antibody (ABIN1387597)

Liu, Cai, Zhang, Liu, Luo, Xing, Du: "Deacetylation of NAT10 by Sirt1 promotes the transition from rRNA biogenesis to autophagy upon energy stress." in: Nucleic acids research, Vol. 46, Issue 18, pp. 9601-9616, (2019) (PubMed).

Liu, Zhang, Liu, Li, Zhang, Li, Liu, Huang, Shen, Gu, Gao, Wu, Wu: "High DEPTOR expression correlates with poor prognosis in patients with esophageal squamous cell carcinoma." in: OncoTargets and therapy, Vol. 8, pp. 3449-55, (2015) (PubMed).

Target Details for DEPTOR

Target: DEPTOR (DEP Domain Containing mTOR-Interacting Protein (DEPTOR))

Alternative Name: Deptor

Background:

Synonyms: DEP.6, DEPDC6, DEP domain-containing mTOR-interacting protein, DEP domain-containing protein 6, DEPTOR

Background: DEPTOR (DEP domain containing MTOR-interacting protein), also known as DEP.6 or DEPDC6 (DEP domain-containing protein 6), is a 409 amino acid protein that negatively regulates mTORC1 and mTORC2 pathways. DEPTOR interacts with FRAP via its PDZ domain, and undergoes post-translational phosphorylation. Containing two DEP domains and one PDZ (DHR) domain, DEPTOR is encoded by a gene that maps to human chromosome 8q24.12. Chromosome 8 consists of nearly 146 million base pairs, encodes over 800 genes and is associated with a variety of diseases and malignancies. Schizophrenia, bipolar disorder, Trisomy 8, Pfeiffer syndrome, congenital hypothyroidism, Waardenburg syndrome and some leukemias and lymphomas are thought to occur as a result of defects in specific genes that map to chromosome 8.

Gene ID: 64798

UniProt: Q8TB45

Pathways: PI3K-Akt Signaling, RTK Signaling, Regulation of Cell Size, BCR Signaling, Warburg Effect