UBE2F antibody (AA 21-120)

Catalog No. ABIN1387600

Product Details anti-UBE2F Antibody

Target: UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))

Binding Specificity: AA 21-120

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2F antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human NCE2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for UBE2F

Target: UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))

Alternative Name: NCE2 (UBE2F Products)

Synonyms: NCE2 antibody, 2510010F15Rik antibody, AI851109 antibody, RGD1307608 antibody, zgc:77005 antibody, ubiquitin conjugating enzyme E2 F (putative) antibody, NEDD8-conjugating enzyme UBE2F antibody, ubiquitin-conjugating enzyme E2F (putative) antibody, ubiquitin conjugating enzyme E2F (putative) S homeolog antibody, UBE2F antibody, Tsp_01616 antibody, ube2f antibody, ube2f.S antibody, Ube2f antibody

Background:

Synonyms: NCE2, NEDD8 carrier protein UBE2F, NEDD8 conjugating enzyme, NEDD8 conjugating enzyme UBE2F, NEDD8 protein ligase UBE2F, NEDD8-conjugating enzyme 2, NEDD8-conjugating enzyme UBE2F, ube2f, UBE2F_HUMAN, Ubiquitin conjugating enzyme E2F, Ubiquitin conjugating enzyme E2F putative, Ubiquitin-conjugating enzyme E2 F.

Background: Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.