Acrosin antibody (AA 201-300)

Catalog No. ABIN1387665

This anti-Acrosin antibody is a Rabbit Polyclonal antibody detecting Acrosin in WB, ELISA, FACS, IF (cc), IF (p), IHC (p) and IHC (fro). Suitable for Human, Rat, Mouse and Goat. This Primary Antibody has been cited in 4+ publications.

Quick Overview for Acrosin antibody (AA 201-300) (ABIN1387665)

Target: Acrosin (ACR)

Reactivity: Human, Rat, Mouse, Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Acrosin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Acrosin Antibody

Binding Specificity: AA 201-300

Cross-Reactivity: Goat, Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Sheep,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Acrosin

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Acrosin antibody (ABIN1387665)

Deng, Zhang, Yu, Wang, Chen, Han, Cheng, Lian, Liu et al.: "Melatonin up-regulates the expression of the GATA-4 transcription factor and increases testosterone secretion from Leydig cells through RORα signaling in an in vitro goat spermatogonial stem cell ..." in: Oncotarget, Vol. 8, Issue 66, pp. 110592-110605, (2017) (PubMed).

Deng, Chen, Wang, Zhang, Tang, Li, Wang, Cheng, Jin, Li, Zhang, Yu, Lian, Liu, Liu: "Melatonin promotes development of haploid germ cells from early developing spermatogenic cells of Suffolk sheep under in vitro condition." in: Journal of pineal research, Vol. 60, Issue 4, pp. 435-47, (2016) (PubMed).

Liang, Cheng, Jiang, He, Wang, Jiang, Hou, Li, Liu, Wang: "The noncircadian function of the circadian Clock gene in the regulation of male fertility." in: Journal of biological rhythms, Vol. 28, Issue 3, pp. 208-17, (2013) (PubMed).

Target Details for Acrosin

Target: Acrosin (ACR)

Alternative Name: Acrosin

Background:

Synonyms: Acrosin, ACR, ACRS

Background: Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome.

Gene ID: 49

UniProt: P10323

Pathways: cAMP Metabolic Process