Acrosin antibody (ACR)

Details for Product anti-ACR Antibody No. ABIN94455
Antigen
Reactivity
Pig (Porcine)
62
20
18
6
2
2
2
Host
Mouse
61
8
1
Clonality (Clone)
Monoclonal ()
Conjugate
This Acrosin antibody is un-conjugated
3
3
3
3
3
3
3
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Western Blotting (WB)
47
21
17
13
10
10
9
2
2
2
1
Options
Immunogen Acid extracts of boar spermatozoa were subjected to hydrophobic chromatography and the pooled fraction with reactivity to N-alpha benzoylarginine-4-nitroanilide was used for immunization.
Clone ACR-2
Isotype IgG1
Specificity The antibody ACR-2 reacts with various forms of porcine acrosin (55, 53, 45 and 35 kDa), a typical serine proteinase with trypsin-like specificity. Acrosin is stored in the acrosome of undamaged spermatozoa.
No Cross-Reactivity Human, Cow (Bovine), Dog (Canine)
Cross-Reactivity (Details) Porcine
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name Acrosin (ACR Antibody Abstract)
Pathways cAMP Metabolic Process
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Product cited in: Ded, Dostalova, Dorosh, Dvorakova-Hortova, Peknicova: "Effect of estrogens on boar sperm capacitation in vitro." in: Reproductive biology and endocrinology : RB&E, Vol. 8, pp. 87, 2010 (PubMed).

Peknicova, Capkova, Geussova, Ivanova, Mollova: "Monoclonal antibodies to intra-acrosomal proteins inhibit gamete binding in vitro." in: Theriogenology, Vol. 56, Issue 2, pp. 211-23, 2001 (PubMed).

Moos, Peknicova, Tesarik: "Protein-protein interactions controlling acrosin release and solubilization during the boar sperm acrosome reaction." in: Biology of reproduction, Vol. 49, Issue 2, pp. 408-15, 1993 (PubMed).

Moos, Peknicova, Tesarik: "Relationship between molecular conversions of acrosin and the progression of exocytosis in the calcium ionophore-induced acrosome reaction." in: Biochimica et biophysica acta, Vol. 1176, Issue 3, pp. 199-207, 1993 (PubMed).

Moos, Tesarik, Leca, Peknicova: "Mechanism of maturation and nature of carbohydrate chains of boar sperm acrosin." in: FEBS letters, Vol. 294, Issue 1-2, pp. 27-30, 1992 (PubMed).

Peknicová, Moos: "Monoclonal antibodies against boar acrosomal antigens labelling undamaged acrosomes of spermatozoa in immunofluorescence test." in: Andrologia, Vol. 22, Issue 5, pp. 427-35, 1991 (PubMed).