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Acrosin antibody

ACR Reactivity: Pig WB, FACS, ICC Host: Mouse Monoclonal ACR-2 unconjugated
Catalog No. ABIN94455
  • Target See all Acrosin (ACR) Antibodies
    Acrosin (ACR)
    Reactivity
    • 32
    • 17
    • 17
    • 16
    • 2
    • 2
    • 2
    • 1
    Pig
    Host
    • 32
    • 2
    • 1
    Mouse
    Clonality
    • 33
    • 2
    Monoclonal
    Conjugate
    • 20
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Acrosin antibody is un-conjugated
    Application
    • 34
    • 18
    • 13
    • 13
    • 12
    • 9
    • 7
    • 4
    • 3
    • 2
    • 2
    • 2
    Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)
    Specificity
    The antibody ACR-2 reacts with various forms of porcine acrosin (55, 53, 45 and 35 kDa), a typical serine proteinase with trypsin-like specificity. Acrosin is stored in the acrosome of undamaged spermatozoa.
    No Cross-Reactivity
    Cow, Dog, Human
    Cross-Reactivity (Details)
    Porcine
    Purification
    Purified by protein-A affinity chromatography.
    Purity
    > 95 % (by SDS-PAGE)
    Immunogen
    Acid extracts of boar spermatozoa were subjected to hydrophobic chromatography and the pooled fraction with reactivity to N-alpha benzoylarginine-4-nitroanilide was used for immunization.
    Clone
    ACR-2
    Isotype
    IgG1
    Top Product
    Discover our top product ACR Primary Antibody
  • Application Notes
    Flow cytometry: Recommended dilution: 1-12 μg/mL. Intracellular staining.
    Immunocytochemistry: Membrane permeabilization (acetone) is essential.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Do not freeze.
  • Ded, Dostalova, Zatecka, Dorosh, Komrskova, Peknicova: "Fluorescent analysis of boar sperm capacitation process in vitro." in: Reproductive biology and endocrinology : RB&E, Vol. 17, Issue 1, pp. 109, (2020) (PubMed).

    Ded, Dostalova, Dorosh, Dvorakova-Hortova, Peknicova: "Effect of estrogens on boar sperm capacitation in vitro." in: Reproductive biology and endocrinology : RB&E, Vol. 8, pp. 87, (2010) (PubMed).

    Peknicova, Capkova, Geussova, Ivanova, Mollova: "Monoclonal antibodies to intra-acrosomal proteins inhibit gamete binding in vitro." in: Theriogenology, Vol. 56, Issue 2, pp. 211-23, (2001) (PubMed).

    Moos, Peknicova, Tesarik: "Protein-protein interactions controlling acrosin release and solubilization during the boar sperm acrosome reaction." in: Biology of reproduction, Vol. 49, Issue 2, pp. 408-15, (1993) (PubMed).

    Moos, Peknicova, Tesarik: "Relationship between molecular conversions of acrosin and the progression of exocytosis in the calcium ionophore-induced acrosome reaction." in: Biochimica et biophysica acta, Vol. 1176, Issue 3, pp. 199-207, (1993) (PubMed).

    Moos, Tesarik, Leca, Peknicova: "Mechanism of maturation and nature of carbohydrate chains of boar sperm acrosin." in: FEBS letters, Vol. 294, Issue 1-2, pp. 27-30, (1992) (PubMed).

    Peknicová, Moos: "Monoclonal antibodies against boar acrosomal antigens labelling undamaged acrosomes of spermatozoa in immunofluorescence test." in: Andrologia, Vol. 22, Issue 5, pp. 427-35, (1991) (PubMed).

  • Target
    Acrosin (ACR)
    Alternative Name
    Acrosin (ACR Products)
    Synonyms
    ACR antibody, MGC130972 antibody, Acro antibody, Acrosin antibody, acrosin antibody, acrosin L homeolog antibody, acrosin prepropeptide antibody, ACR antibody, acr.L antibody, Acr antibody
    Background
    Acrosin,Acrosin is a serine proteinase expressed in the acrosome of mature spermatozoa. This enzyme facilitates penetration of the sperm through the zona pellucida of the ovum.,ACR, FBP53
    Gene ID
    397098
    UniProt
    P08001
    Pathways
    cAMP Metabolic Process
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