HNF4A antibody (pSer303)

Catalog No. ABIN1387865

The Rabbit Polyclonal anti-HNF4A antibody has been validated for WB, ELISA, IHC (p), IF (cc), IF (p) and IHC (fro). It is suitable to detect HNF4A in samples from Mouse and Zebrafish (Danio rerio).

Quick Overview for HNF4A antibody (pSer303) (ABIN1387865)

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Reactivity: Mouse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HNF4A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-HNF4A Antibody

Binding Specificity: pSer303

Cross-Reactivity: Mouse, Zebrafish (Danio rerio)

Predicted Reactivity: Human,Rat,Cow,Pig,Horse,Chicken,Rabbit,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human HNF4 alpha around the phosphorylation site of Ser303

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for HNF4A

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Alternative Name: HNF4

Background:

Synonyms: HNF4Ser303, Hepatic nuclear factor 4 alpha, Hepatocyte nuclear factor 4 alpha, Hepatocyte nuclear factor 4, HNF 4 alpha, HNF 4, HNF4, HNF4A, HNF4a7, HNF4a8, HNF4a9, Hnf4alpha, MODY 1, MODY, MODY1, NR2A1, NR2A21, Nuclear receptor subfamily 2 group A member 1, TCF 14, TCF, TCF14, Tcf4, Transcription factor 14, hepatic nuclear factor, Transcription factor 14, Transcription factor HNF 4, Transcription factor HNF4, HNF4A_HUMAN.

Background: The protein encoded by the HNF4 gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non insulin dependent diabetes mellitus type I. At least three different transcript variants encoding three different isoforms have been found for this gene.

Gene ID: 3172

Pathways: AMPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process